SIRT3 SNPs validation in 640 individuals, functional analyses and new insights into SIRT3 stability

• Verfasst von: Dransfeld, Christian-Lars [VerfasserIn]
• Verfasst von: Alborzinia, Hamed [VerfasserIn]
• Verfasst von: Wölfl, Stefan [VerfasserIn]
• Verfasst von: Mahlknecht, Ulrich [VerfasserIn]
• Titel: SIRT3 SNPs validation in 640 individuals, functional analyses and new insights into SIRT3 stability
• Verf.angabe: Christian-Lars Dransfeld, Hamed Alborzinia, Stefan Wölfl and Ulrich Mahlknecht
• E-Jahr: 2010
• Jahr: April 1, 2010
• Umfang: 6 S.
• Fussnoten: Gesehen am 16.02.2023
• Titel Quelle: Enthalten in: International journal of oncology
• Ort Quelle: Athens : Spandidos Publ., 2001
• Jahr Quelle: 2010
• Band/Heft Quelle: 36(2010), 4 vom: Apr., Seite 955-960
• ISSN Quelle: 1791-2423
• DOI: doi:10.3892/ijo_00000574
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 183652594X

Abstract

Sirtuins are critical players within multiple cellular pathways such as stress response, apoptosis and energy metabolism. They are associated with metabolic and degenerative diseases, the pathogenesis of cancer and are key elements in the regulation of cellular life span. From within the 7 known human sirtuins, SIRT3 recently stepped out of the shadow of SIRT1 showing strong effects on stress response, apoptosis, cell cycle and energy metabolism, mimicking effects of caloric restriction. We have identified two non-synonymous human SIRT3 SNPs and evaluated their impact on SIRT3 activity and stability. We assessed their influence on cellular energy metabolism in relation to SIRT1 and identified SIRT3 to increase cellular respiration by 80% when compared to SIRT1, which increased cellular respiration by only 30%.