| Online-Ressource |
Verfasst von: | Dreher, Yannik [VerfasserIn]  |
| Fichtler, Julius [VerfasserIn]  |
| Karfusehr, Christoph [VerfasserIn]  |
| Jahnke, Kevin [VerfasserIn]  |
| Xin, Yang [VerfasserIn]  |
| Keller, Adrian [VerfasserIn]  |
| Göpfrich, Kerstin [VerfasserIn]  |
Titel: | Genotype-phenotype mapping with polyominos made from DNA origami tiles |
Verf.angabe: | Yannik Dreher, Julius Fichtler, Christoph Karfusehr, Kevin Jahnke, Yang Xin, Adrian Keller and Kerstin Göpfrich |
E-Jahr: | 2022 |
Jahr: | 20 December 2022 |
Umfang: | 9 S. |
Fussnoten: | Online veröffentlicht: 10. September 2022, Artikelversion: 20. Dezember 2022 ; Gesehen am 20.02.2023 |
Titel Quelle: | Enthalten in: Biophysical journal |
Ort Quelle: | Cambridge, Mass. : Cell Press, 1960 |
Jahr Quelle: | 2022 |
Band/Heft Quelle: | 121(2022), 24, Seite 4840-4848 |
ISSN Quelle: | 1542-0086 |
Abstract: | The correlation between genetic information and characteristics of a living cell - its genotype and its phenotype - constitutes the basis of genetics. Here, we experimentally realize a primitive form of genotype-phenotype mapping with DNA origami. The DNA origami can polymerize into two-dimensional lattices (phenotype) via blunt-end stacking facilitated by edge staples at the seam of the planar DNA origami. There are 80 binding positions for edge staples, which allow us to translate an 80-bit long binary code (genotype) onto the DNA origami. The presence of an edge staple thus corresponds to a ‘‘1’’ and its absence to a ‘‘0.’’ The interactions of our DNA-based system can be reproduced by a polyomino model. Polyomino growth simulations qualitatively reproduce our experimental results. We show that not only the absolute number of base stacks but also their sequence position determine the cluster size and correlation length of the orientation of single DNA origami within the cluster. Importantly, the mutation of a few bits can result in major morphology changes of the DNA origami cluster, while more often, major sequence changes have no impact. Our experimental realization of a correlation between binary information (‘‘genotype’’) and cluster morphology (‘‘phenotype’’) thus reproduces key properties of genotype-phenotype maps known from living systems. |
DOI: | doi:10.1016/j.bpj.2022.09.006 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.bpj.2022.09.006 |
| Volltext: https://linkinghub.elsevier.com/retrieve/pii/S0006349522007342 |
| DOI: https://doi.org/10.1016/j.bpj.2022.09.006 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1837127115 |
Verknüpfungen: | → Zeitschrift |
Genotype-phenotype mapping with polyominos made from DNA origami tiles / Dreher, Yannik [VerfasserIn]; 20 December 2022 (Online-Ressource)