The antioxidant Rutin counteracts the pathological impact of α-synuclein on the enteric nervous system in vitro

• Verfasst von: Christmann, Anne [VerfasserIn]
• Verfasst von: Gries, Manuela [VerfasserIn]
• Verfasst von: Scholz, Patrik [VerfasserIn]
• Verfasst von: Stahr, Pascal-Lothar [VerfasserIn]
• Verfasst von: Law, Jessica Ka Yan [VerfasserIn]
• Verfasst von: Schulte, Steven [VerfasserIn]
• Verfasst von: Martin, Monika [VerfasserIn]
• Verfasst von: Lilischkis, Rainer [VerfasserIn]
• Verfasst von: Ingebrandt, Sven [VerfasserIn]
• Verfasst von: Keck, Cornelia M. [VerfasserIn]
• Verfasst von: Schäfer, Karl Herbert [VerfasserIn]
• Titel: The antioxidant Rutin counteracts the pathological impact of α-synuclein on the enteric nervous system in vitro
• Verf.angabe: Anne Christmann, Manuela Gries, Patrik Scholz, Pascal L. Stahr, Jessica Ka Yan Law, Steven Schulte, Monika Martin, Rainer Lilischkis, Sven Ingebrandt, Cornelia M. Keck, Karl-Herbert Schäfer
• Jahr: 2022
• Umfang: 20 S.
• Fussnoten: Veröffentlicht von De Gruyter 29. September 2021 ; Gesehen am 21.02.2023
• Titel Quelle: Enthalten in: Biological chemistry
• Ort Quelle: Berlin [u.a.] : de Gruyter, 1996
• Jahr Quelle: 2022
• Band/Heft Quelle: 403(2022), 1, Seite 103-122
• ISSN Quelle: 1437-4315
• DOI: doi:10.1515/hsz-2021-0259
• Datenträger: Online-Ressource
• Sprache: eng
• Sonstige Nr.: Grant number: 03FH019I3
• Sach-SW: A53T-α-synuclein
• Sach-SW: enteric nervous system
• Sach-SW: natural antioxidants
• Sach-SW: oxidative stress
• Sach-SW: Parkinson’s disease
• Sach-SW: Rutin nanocrystals
• K10plus-PPN: 1837226644

Abstract

Motoric disturbances in Parkinson’s disease (PD) derive from the loss of dopaminergic neurons in the substantia nigra. Intestinal dysfunctions often appear long before manifestation of neuronal symptoms, suggesting a strong correlation between gut and brain in PD. Oxidative stress is a key player in neurodegeneration causing neuronal cell death. Using natural antioxidative flavonoids like Rutin, might provide intervening strategies to improve PD pathogenesis. To explore the potential effects of micro (mRutin) compared to nano Rutin (nRutin) upon the brain and the gut during PD, its neuroprotective effects were assessed using an in vitro PD model. Our results demonstrated that Rutin inhibited the neurotoxicity induced by A53T α -synuclein (Syn) administration by decreasing oxidized lipids and increasing cell viability in both, mesencephalic and enteric cells. For enteric cells, neurite outgrowth, number of synaptic vesicles, and tyrosine hydroxylase positive cells were significantly reduced when treated with Syn. This could be reversed by the addition of Rutin. nRutin revealed a more pronounced result in all experiments. In conclusion, our study shows that Rutin, especially the nanocrystals, are promising natural compounds to protect neurons from cell death and oxidative stress during PD. Early intake of Rutin may provide a realizable option to prevent or slow PD pathogenesis.