O-ring aortic banding versus traditional transverse aortic constriction for modeling pressure overload-induced cardiac hypertrophy

• Verfasst von: Schmiedel, Nesrin [VerfasserIn]
• Verfasst von: Remes, Anca [VerfasserIn]
• Verfasst von: Valadan, Mohsen [VerfasserIn]
• Verfasst von: Hille, Susanne [VerfasserIn]
• Verfasst von: Matzen, Andrea [VerfasserIn]
• Verfasst von: Frank, Derk [VerfasserIn]
• Verfasst von: Frey, Norbert [VerfasserIn]
• Verfasst von: Lehmann, Lorenz [VerfasserIn]
• Verfasst von: Müller, Oliver J. [VerfasserIn]
• Titel: O-ring aortic banding versus traditional transverse aortic constriction for modeling pressure overload-induced cardiac hypertrophy
• Verf.angabe: Nesrin Schmiedel, Anca Remes, Mohsen Valadan, Susanne Hille, Andrea Matzen, Derk Frank, Norbert Frey, Lorenz Lehmann, Oliver J. Müller
• E-Jahr: 2022
• Jahr: October 6th, 2022
• Umfang: 1 Online-Ressource (1 Videodatei, 9:23 min)
• Umfang: 12 S.
• Illustrationen: farbig
• Fussnoten: Enthält auch Textversion ; Wissenschaftlicher Film. Deutschland. 2022
• Titel Quelle: Enthalten in: JoVE. Video journal
• Ort Quelle: [Erscheinungsort nicht ermittelbar] : [Verlag nicht ermittelbar], 2006
• Jahr Quelle: 2022
• Band/Heft Quelle: (2022), 188, Artikel-ID e64455, Seite 1-12
• ISSN Quelle: 1940-087X
• DOI: doi:10.3791/64455
• Datenträger: Online-Ressource
• Dokumenttyp: Film
• Sprache: eng
• K10plus-PPN: 1837296669

Abstract

Aortic banding in mice is one of the most commonly used experimental models for cardiac pressure overload-induced cardiac hypertrophy and the induction of heart failure. The previously used technique is based on a threaded suture around the aortic arch tied over a blunted 27 G needle to create stenosis. This method depends on the surgeon manually tightening the thread and, thus, leads to high variance in the diameter size. A newly refined method described by Melleby et al. promises less variance and more reproducibility after surgery. The new technique, o-ring- aortic banding (ORAB), uses a non-slip rubber ring instead of a suture with a thread, resulting in reduced variation in pressure overload and reproducible phenotypes of cardiac hypertrophy. During surgery, the o-ring is placed between the brachiocephalic and left carotid arteries. Successful constriction is confirmed by echocardiography. After 1 day, correct placement of the ring results in an increased flow velocity in the transverse aorta over the o-ring-induced stenosis. After 2 weeks, impaired cardiac function is proven by decreased ejection fraction and increased wall thickness. Importantly, besides less variance in the diameter size, ORAB is associated with lower intra- and post-operative mortality rates compared with transverse aortic constriction (TAC). Thus, ORAB represents a superior method to the commonly used TAC surgery, resulting in more reproducible results and a possible reduction in the number of animals needed.