| Online-Ressource |
Verfasst von: | Ebert, Antje [VerfasserIn]  |
| Laußmann, Mareike [VerfasserIn]  |
| Wegehingel, Sabine [VerfasserIn]  |
| Kaderali, Lars [VerfasserIn]  |
| Erfle, Holger [VerfasserIn]  |
| Reichert, Jürgen [VerfasserIn]  |
| Lechner, Johannes [VerfasserIn]  |
| Beer, Hans-Dietmar [VerfasserIn]  |
| Pepperkok, Rainer [VerfasserIn]  |
| Nickel, Walter [VerfasserIn]  |
Titel: | Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion |
Verf.angabe: | Antje D. Ebert, Mareike Laußmann, Sabine Wegehingel, Lars Kaderali, Holger Erfle, Jürgen Reichert, Johannes Lechner, Hans-Dietmar Beer, Rainer Pepperkok and Walter Nickel |
E-Jahr: | 2010 |
Jahr: | 10 May 2010 |
Umfang: | 14 S. |
Fussnoten: | Gesehen am 27.02.2023 |
Titel Quelle: | Enthalten in: Traffic |
Ort Quelle: | Oxford : Wiley-Blackwell, 2000 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 11(2010), 6, Seite 813-826 |
ISSN Quelle: | 1600-0854 |
Abstract: | Fibroblast growth factor 2 (FGF2) is a potent mitogen that is exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across plasma membranes, a process that depends on the phosphoinositide phosphatidylinositol 4,5-biphosphate (PI(4,5)P2) at the inner leaflet as well as heparan sulfate proteoglycans at the outer leaflet of plasma membranes; however, additional core and regulatory components of the FGF2 export machinery have remained elusive. Here, using a highly effective RNAi screening approach, we discovered Tec kinase as a novel factor involved in unconventional secretion of FGF2. Tec kinase does not affect FGF2 secretion by an indirect mechanism, but rather forms a heterodimeric complex with FGF2 resulting in phosphorylation of FGF2 at tyrosine 82, a post-translational modification shown to be essential for FGF2 membrane translocation to cell surfaces. Our findings suggest a crucial role for Tec kinase in regulating FGF2 secretion under various physiological conditions and, therefore, provide a new perspective for the development of a novel class of antiangiogenic drugs targeting the formation of the FGF2/Tec complex. |
DOI: | doi:10.1111/j.1600-0854.2010.01059.x |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/j.1600-0854.2010.01059.x |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0854.2010.01059.x |
| DOI: https://doi.org/10.1111/j.1600-0854.2010.01059.x |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | FGF2 |
| membrane translocation |
| non-classical export |
| phosphorylation |
| RNAi screening |
| Tec kinase |
| unconventional protein secretion |
K10plus-PPN: | 183751237X |
Verknüpfungen: | → Zeitschrift |
Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion / Ebert, Antje [VerfasserIn]; 10 May 2010 (Online-Ressource)