| |  Online-Ressource |
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| Verfasst von: | Ebert, Antje [VerfasserIn]  |
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| | Laußmann, Mareike [VerfasserIn] |
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| | Wegehingel, Sabine [VerfasserIn] |
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| | Kaderali, Lars [VerfasserIn] |
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| | Erfle, Holger [VerfasserIn] |
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| | Reichert, Jürgen [VerfasserIn] |
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| | Lechner, Johannes [VerfasserIn] |
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| | Beer, Hans-Dietmar [VerfasserIn] |
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| | Pepperkok, Rainer [VerfasserIn] |
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| | Nickel, Walter [VerfasserIn] |
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| Titel: | Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion |
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| Verf.angabe: | Antje D. Ebert, Mareike Laußmann, Sabine Wegehingel, Lars Kaderali, Holger Erfle, Jürgen Reichert, Johannes Lechner, Hans-Dietmar Beer, Rainer Pepperkok and Walter Nickel |
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| E-Jahr: | 2010 |
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| Jahr: | 10 May 2010 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 27.02.2023 |
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| Titel Quelle: | Enthalten in: Traffic |
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| Ort Quelle: | Oxford : Wiley-Blackwell, 2000 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 11(2010), 6, Seite 813-826 |
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| ISSN Quelle: | 1600-0854 |
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| Abstract: | Fibroblast growth factor 2 (FGF2) is a potent mitogen that is exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across plasma membranes, a process that depends on the phosphoinositide phosphatidylinositol 4,5-biphosphate (PI(4,5)P2) at the inner leaflet as well as heparan sulfate proteoglycans at the outer leaflet of plasma membranes; however, additional core and regulatory components of the FGF2 export machinery have remained elusive. Here, using a highly effective RNAi screening approach, we discovered Tec kinase as a novel factor involved in unconventional secretion of FGF2. Tec kinase does not affect FGF2 secretion by an indirect mechanism, but rather forms a heterodimeric complex with FGF2 resulting in phosphorylation of FGF2 at tyrosine 82, a post-translational modification shown to be essential for FGF2 membrane translocation to cell surfaces. Our findings suggest a crucial role for Tec kinase in regulating FGF2 secretion under various physiological conditions and, therefore, provide a new perspective for the development of a novel class of antiangiogenic drugs targeting the formation of the FGF2/Tec complex. |
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| DOI: | doi:10.1111/j.1600-0854.2010.01059.x |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1111/j.1600-0854.2010.01059.x |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0854.2010.01059.x |
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| | DOI: https://doi.org/10.1111/j.1600-0854.2010.01059.x |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | FGF2 |
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| | membrane translocation |
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| | non-classical export |
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| | phosphorylation |
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| | RNAi screening |
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| | Tec kinase |
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| | unconventional protein secretion |
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| K10plus-PPN: | 183751237X |
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| Verknüpfungen: | → Zeitschrift |
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Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion / Ebert, Antje [VerfasserIn]; 10 May 2010 (Online-Ressource)
