| |  Online-Ressource |
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| Verfasst von: | Kane, Nicole M. [VerfasserIn]  |
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| | Nowrouzi, Ali [VerfasserIn] |
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| | Mukherjee, Sayandip [VerfasserIn] |
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| | Blundell, Michael P [VerfasserIn] |
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| | Greig, Jenny A [VerfasserIn] |
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| | Lee, Wai Kwong [VerfasserIn] |
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| | Houslay, Miles D [VerfasserIn] |
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| | Milligan, Graeme [VerfasserIn] |
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| | Mountford, Joanne C [VerfasserIn] |
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| | Kalle, Christof von [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Thrasher, Adrian J [VerfasserIn] |
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| | Baker, Andrew H [VerfasserIn] |
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| Titel: | Lentivirus-mediated reprogramming of somatic cells in the absence of transgenic transcription factors |
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| Verf.angabe: | Nicole M Kane, Ali Nowrouzi, Sayandip Mukherjee, Michael P Blundell, Jenny A Greig, Wai Kwong Lee, Miles D Houslay, Graeme Milligan, Joanne C Mountford, Christof von Kalle, Manfred Schmidt, Adrian J Thrasher and Andrew H Baker |
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| E-Jahr: | 2010 |
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| Jahr: | 14 December 2016 |
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| Umfang: | 7 S. |
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| Fussnoten: | Gesehen am 01.03.2023 |
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| Titel Quelle: | Enthalten in: Molecular therapy |
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| Ort Quelle: | Amsterdam : Elsevier, 2000 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 18(2010), 12, Seite 2139-2145 |
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| ISSN Quelle: | 1525-0024 |
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| Abstract: | Retroviral vectors remain the most efficient and widely applied system for induction of pluripotency. However, mutagenic effects have been documented in both laboratory and clinical gene therapy studies, principally as a result of dysregulated host gene expression in the proximity of defined integration sites. Here, we report that cells with characteristics of pluripotent stem cells can be produced from normal human fibroblasts in the absence of reprogramming transcription factors (TFs) during lentiviral (LV) vector-mediated gene transfer. This occurred via induced alterations in host gene and microRNA (miRNA) expression and detrimental changes in karyotype. These findings demonstrate that vector-induced genotoxicity may alone play a role in somatic cell reprogramming derivation and urges caution when using integrating vectors in this setting. Clearer understanding of this process may additionally reveal novel insights into reprogramming pathways. |
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| DOI: | doi:10.1038/mt.2010.231 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/mt.2010.231 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S152500161630908X |
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| | DOI: https://doi.org/10.1038/mt.2010.231 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1837806055 |
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| Verknüpfungen: | → Zeitschrift |
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Lentivirus-mediated reprogramming of somatic cells in the absence of transgenic transcription factors / Kane, Nicole M. [VerfasserIn]; 14 December 2016 (Online-Ressource)
