Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Kilic, Annett [VerfasserIn]   i
 Klose, Sven [VerfasserIn]   i
 Dobberstein, Bernhard [VerfasserIn]   i
 Knust, Elisabeth [VerfasserIn]   i
 Kapp, Katja [VerfasserIn]   i
Titel:The Drosophila Crumbs signal peptide is unusually long and is a substrate for signal peptide peptidase
Verf.angabe:Annett Kilic, Sven Klose, Bernhard Dobberstein, Elisabeth Knust, Katja Kapp
E-Jahr:2010
Jahr:1 March 2010
Umfang:13 S.
Fussnoten:Gesehen am 09.03.2023
Titel Quelle:Enthalten in: European journal of cell biology
Ort Quelle:München : Elsevier, 1998
Jahr Quelle:2010
Band/Heft Quelle:89(2010), 6 vom: Juni, Seite 449-461
ISSN Quelle:1618-1298
Abstract:N-terminal signal sequences mediate nascent protein targeting to and protein insertion into the membrane of the endoplasmic reticulum. They are typically 15-30 amino acid residues long with a core hydrophobic region flanked by an N-terminal (n-) and a C-terminal region. Following cleavage by signal peptidase, some of the resulting signal peptides are further processed by signal peptide peptidase (SPP) and fragments are liberated into the cytosol. Such fragments can have independent, post-targeting functions affecting diverse cellular processes. We show that Drosophila melanogaster Crumbs, a transmembrane protein controlling cell polarity and morphogenesis, is synthesized with an 83 residues-long signal sequence. To our knowledge, this is currently the longest signal sequence described for an eukaryotic protein. The unusual length is caused by an extended n-region, but the extension does neither affect protein targeting nor signal sequence cleavage. The signal sequence is cleaved off and the resulting signal peptide, SPCrb, is proteolytically processed by SPP, thus representing the first substrate described for the Drosophila enzyme. We further show that signal peptide fragments can be degraded by the proteasome. Expression of transgenes encoding tagged variants of Crumbs in Drosophila embryos suggests that the signal peptide is short-lived in vivo. Our findings support a model suggesting that besides generating fragments with post-targeting functions, SPP-mediated processing is the first step in the degradation of signal peptides.
DOI:doi:10.1016/j.ejcb.2010.02.001
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.ejcb.2010.02.001
 Volltext: https://www.sciencedirect.com/science/article/pii/S0171933510000312
 DOI: https://doi.org/10.1016/j.ejcb.2010.02.001
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Crumbs
 ER targeting
 signal peptide
 signal peptide peptidase
K10plus-PPN:1838746951
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/69050083   QR-Code
zum Seitenanfang