| Online-Ressource |
Verfasst von: | Lanz, Tobias [VerfasserIn]  |
| Opitz, Christiane [VerfasserIn]  |
| Ho, Peggy P. [VerfasserIn]  |
| Agrawal, Ankur [VerfasserIn]  |
| Lutz, Christian [VerfasserIn]  |
| Weller, Michael [VerfasserIn]  |
| Mellor, Andrew L. [VerfasserIn]  |
| Steinman, Lawrence [VerfasserIn]  |
| Wick, Wolfgang [VerfasserIn]  |
| Platten, Michael [VerfasserIn]  |
Titel: | Mouse mesenchymal stem cells suppress antigen-specific TH cell immunity independent of indoleamine 2,3-dioxygenase 1 (IDO1) |
Verf.angabe: | Tobias V. Lanz, Christiane A. Opitz, Peggy P. Ho, Ankur Agrawal, Christian Lutz, Michael Weller, Andrew L. Mellor, Lawrence Steinman, Wolfgang Wick, Michael Platten |
E-Jahr: | 2010 |
Jahr: | May 2010 |
Umfang: | 12 S. |
Fussnoten: | Online veröffentlicht: 3 Nov 2009 ; Gesehen am 10.03.2023 |
Titel Quelle: | Enthalten in: Stem Cells and Development |
Ort Quelle: | Larchmont, NY : Liebert, 2004 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 19(2010), 5 vom: Mai, Seite 657-668 |
ISSN Quelle: | 1557-8534 |
Abstract: | Due to their immunosuppressive properties, human mesenchymal stem cells (hMSC) represent a promising tool for cell-based therapies of autoimmune diseases such as multiple sclerosis (MS). Mouse MSC (mMSC) have been used extensively to characterize and optimize route of administration, motility, cellular targets, and immunosuppressive mechanisms in mouse models of autoimmune diseases, such as experimental autoimmune encephalomyelitis (EAE). Tryptophan (trp) catabolism by indolamine-2,3-dioxygenase 1 (IDO1) is a chief endogenous metabolic pathway that tightly regulates unwanted immune responses through depletion of trp and generation of immunosuppressive kynurenines (kyn). IDO1 activity contributes to the immunosuppressive phenotype of hMSC. Here, we demonstrate that although IDO1 is inducible in bone marrow-derived mMSC by proinflammatory stimuli such as interferon-g (IFN-g) and ligands of toll-like receptors (TLR), it does not lead to catabolism of trp in vitro. This failure to catabolize trp is not due to defective TLR signaling as demonstrated by induction of interleukin 6 (IL-6) by TLR activation. While mMSC suppressed the activation of antigen-specific myelin oligodendrocyte glycoprotein (MOG)-reactive T-cell receptor (TCR) transgenic T-helper (TH) cells in co-culture, neither pharmacologic inhibition nor genetic ablation of IDO1 reversed this suppressive effect. Finally, systemic administration of both, IDO1-proficient and phenotypically identical IDO1-deficient mMSC, equally resulted in amelioration of EAE. mMSC, unlike hMSC, do not display IDO1-mediated suppression of antigen-specific T-cell responses. |
DOI: | doi:10.1089/scd.2009.0385 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1089/scd.2009.0385 |
| Volltext: https://www.liebertpub.com/doi/10.1089/scd.2009.0385 |
| DOI: https://doi.org/10.1089/scd.2009.0385 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1838856986 |
Verknüpfungen: | → Zeitschrift |
Mouse mesenchymal stem cells suppress antigen-specific TH cell immunity independent of indoleamine 2,3-dioxygenase 1 (IDO1) / Lanz, Tobias [VerfasserIn]; May 2010 (Online-Ressource)