Mutual regulation of cyclin-dependent kinase and the mitotic exit network

• Verfasst von: König, Cornelia [VerfasserIn]
• Verfasst von: Maekawa, Hiromi [VerfasserIn]
• Verfasst von: Schiebel, Elmar [VerfasserIn]
• Titel: Mutual regulation of cyclin-dependent kinase and the mitotic exit network
• Verf.angabe: Cornelia König, Hiromi Maekawa, and Elmar Schiebel
• E-Jahr: 2010
• Jahr: February 01 2010
• Umfang: 18 S.
• Fussnoten: Gesehen am 16.03.2023
• Titel Quelle: Enthalten in: The journal of cell biology
• Ort Quelle: New York, NY : Rockefeller Univ. Press, 1962
• Jahr Quelle: 2010
• Band/Heft Quelle: 188(2010), 3, Seite 351-368
• ISSN Quelle: 1540-8140
• DOI: doi:10.1083/jcb.200911128
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1839372060

Abstract

The mitotic exit network (MEN) is a spindle pole body (SPB)-associated, GTPase-driven signaling cascade that controls mitotic exit. The inhibitory Bfa1-Bub2 GTPase-activating protein (GAP) only associates with the daughter SPB (dSPB), raising the question as to how the MEN is regulated on the mother SPB (mSPB). Here, we show mutual regulation of cyclin-dependent kinase 1 (Cdk1) and the MEN. In early anaphase Cdk1 becomes recruited to the mSPB depending on the activity of the MEN kinase Cdc15. Conversely, Cdk1 negatively regulates binding of Cdc15 to the mSPB. In addition, Cdk1 phosphorylates the Mob1 protein to inhibit the activity of Dbf2-Mob1 kinase that regulates Cdc14 phosphatase. Our data revise the understanding of the spatial regulation of the MEN. Although MEN activity in the daughter cells is controlled by Bfa1-Bub2, Cdk1 inhibits MEN activity at the mSPB. Consistent with this model, only triple mutants that lack BUB2 and the Cdk1 phosphorylation sites in Mob1 and Cdc15 show mitotic exit defects.