| |  Online-Ressource |
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| Verfasst von: | Casadonte, Rita [VerfasserIn]  |
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| | Kriegsmann, Jörg [VerfasserIn] |
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| | Kriegsmann, Mark [VerfasserIn] |
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| | Kriegsmann, Katharina [VerfasserIn] |
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| | Torcasio, Roberta [VerfasserIn] |
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| | Gallo Cantafio, Maria Eugenia [VerfasserIn] |
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| | Viglietto, Giuseppe [VerfasserIn] |
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| | Amodio, Nicola [VerfasserIn] |
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| Titel: | A comparison of different sample processing protocols for MALDI imaging mass spectrometry analysis of formalin-fixed multiple myeloma cells |
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| Verf.angabe: | Rita Casadonte, Jörg Kriegsmann, Mark Kriegsmann, Katharina Kriegsmann, Roberta Torcasio, Maria Eugenia Gallo Cantafio, Giuseppe Viglietto and Nicola Amodio |
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| E-Jahr: | 2023 |
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| Jahr: | 3 February 2023 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 20.03.2023 |
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| Titel Quelle: | Enthalten in: Cancers |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 15(2023), 3 vom: Feb., Artikel-ID 974, Seite 1-13 |
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| ISSN Quelle: | 2072-6694 |
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| Abstract: | Sample processing of formalin-fixed specimens constitutes a major challenge in molecular profiling efforts. Pre-analytical factors such as fixative temperature, dehydration, and embedding media affect downstream analysis, generating data dependent on technical processing rather than disease state. In this study, we investigated two different sample processing methods, including the use of the cytospin sample preparation and automated sample processing apparatuses for proteomic analysis of multiple myeloma (MM) cell lines using imaging mass spectrometry (IMS). In addition, two sample-embedding instruments using different reagents and processing times were considered. Three MM cell lines fixed in 4% paraformaldehyde were either directly centrifuged onto glass slides using cytospin preparation techniques or processed to create paraffin-embedded specimens with an automatic tissue processor, and further cut onto glass slides for IMS analysis. The number of peaks obtained from paraffin-embedded samples was comparable between the two different sample processing instruments. Interestingly, spectra profiles showed enhanced ion yield in cytospin compared to paraffin-embedded samples along with high reproducibility compared to the sample replicate. |
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| DOI: | doi:10.3390/cancers15030974 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cancers15030974 |
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| | Volltext: https://www.mdpi.com/2072-6694/15/3/974 |
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| | DOI: https://doi.org/10.3390/cancers15030974 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | cytospin |
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| | FFPE |
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| | imaging mass spectrometry |
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| | MALDI |
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| | sample preparation |
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| K10plus-PPN: | 1839570385 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ comparison of different sample processing protocols for MALDI imaging mass spectrometry analysis of formalin-fixed multiple myeloma cells / Casadonte, Rita [VerfasserIn]; 3 February 2023 (Online-Ressource)
