| |  Online-Ressource |
|---|
| Verfasst von: | Erez, Elinor [VerfasserIn]  |
|---|
| | Stjepanović, Goran [VerfasserIn] |
|---|
| | Zelazny, Adrian M. [VerfasserIn] |
|---|
| | Brügger, Britta [VerfasserIn] |
|---|
| | Sinning, Irmgard [VerfasserIn] |
|---|
| | Bibi, Eitan [VerfasserIn] |
|---|
| Titel: | Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo |
|---|
| Verf.angabe: | Elinor Erez, Goran Stjepanovic, Adrian M. Zelazny, Britta Brugger, Irmgard Sinning, and Eitan Bibi |
|---|
| E-Jahr: | 2010 |
|---|
| Jahr: | 18 October 2010 |
|---|
| Umfang: | 7 S. |
|---|
| Fussnoten: | Available online 18 October 2010, version of record 4 January 2021 ; Gesehen am 20.03.2023 |
|---|
| Titel Quelle: | Enthalten in: The journal of biological chemistry |
|---|
| Ort Quelle: | Bethesda, Md. : ASBMB Publications, 1905 |
|---|
| Jahr Quelle: | 2010 |
|---|
| Band/Heft Quelle: | 285(2010), 52 vom: Dez., Seite 40508-40514 |
|---|
| ISSN Quelle: | 1083-351X |
|---|
| Abstract: | The mechanism underlying the interaction of the Escherichia coli signal recognition particle receptor FtsY with the cytoplasmic membrane has been studied in detail. Recently, we proposed that FtsY requires functional interaction with inner membrane lipids at a late stage of the signal recognition particle pathway. In addition, an essential lipid-binding α-helix was identified in FtsY of various origins. Theoretical considerations and in vitro studies have suggested that it interacts with acidic lipids, but this notion is not yet fully supported by in vivo experimental evidence. Here, we present an unbiased genetic clue, obtained by serendipity, supporting the involvement of acidic lipids. Utilizing a dominant negative mutant of FtsY (termed NG), which is defective in its functional interaction with lipids, we screened for E. coli genes that suppress the negative dominant phenotype. In addition to several unrelated phenotype-suppressor genes, we identified pgsA, which encodes the enzyme phosphatidylglycerophosphate synthase (PgsA). PgsA is an integral membrane protein that catalyzes the committed step to acidic phospholipid synthesis, and we show that its overexpression increases the contents of cardiolipin and phosphatidylglycerol. Remarkably, expression of PgsA also stabilizes NG and restores its biological function. Collectively, our results strongly support the notion that FtsY functionally interacts with acidic lipids. |
|---|
| DOI: | doi:10.1074/jbc.M110.140921 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1074/jbc.M110.140921 |
|---|
| | Volltext: https://www.sciencedirect.com/science/article/pii/S0021925819762176 |
|---|
| | DOI: https://doi.org/10.1074/jbc.M110.140921 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Acidic Lipids |
|---|
| | ffh |
|---|
| | ftsy |
|---|
| | Membrane Lipids |
|---|
| | Membrane Proteins |
|---|
| | Phosphatidylglycerol |
|---|
| | Protein Targeting |
|---|
| | Protein Translocation |
|---|
| | Signal Recognition Particle |
|---|
| | SRP |
|---|
| K10plus-PPN: | 1839572787 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo / Erez, Elinor [VerfasserIn]; 18 October 2010 (Online-Ressource)
