An additional long-term mechanism of NF-κB regulation after cytokine treatment in a human hepatoma cell line

• Verfasst von: Kohlhof-Meinecke, Patricia [VerfasserIn]
• Titel: An additional long-term mechanism of NF-κB regulation after cytokine treatment in a human hepatoma cell line
• Verf.angabe: Patricia Kohlhof
• E-Jahr: 2010
• Jahr: 23 September 2010
• Umfang: 11 S.
• Fussnoten: Gesehen am 21.03.2023
• Titel Quelle: Enthalten in: Virchows Archiv
• Ort Quelle: Berlin : Springer, 1847
• Jahr Quelle: 2010
• Band/Heft Quelle: 457(2010), 5, Seite 585-595
• ISSN Quelle: 1432-2307
• DOI: doi:10.1007/s00428-010-0970-0
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: HepG2 cells
• Sach-SW: IL-1β
• Sach-SW: Inflammatory cytokines
• Sach-SW: NF-κB degradation
• Sach-SW: NF-κB regulation
• Sach-SW: NF-κB synthesis
• Sach-SW: Proteasomal degradation
• Sach-SW: TNF-α
• K10plus-PPN: 1839642920

Abstract

The eukaryotic transcription factor nuclear factor-κB (NF-κB) is mainly involved in the regulation of immune response and inflammation. A prolonged activation of NF-κB has been reported in context with chronic diseases. What leads to a prolongation of NF-κB activity is not well understood. Here, an increase in total intracellular NF-κB protein and mRNA levels as well as a temporary colocalization of NF-κB with proteasomes in human hepatocytes after treatment with TNF-α or IL-1β is reported. This indicates that beside an instantaneous activation of NF-κB and partly autoregulated inactivation by breakdown and synthesis of the inhibitors of NF-κB (IκBs), there are also mechanisms for a long-term regulation by de novo-synthesis and degradation of NF-κB protein.