| |  Online-Ressource |
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| Verfasst von: | Triller, Gianna [VerfasserIn]  |
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| | Vlachou, Evi P. [VerfasserIn] |
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| | Hashemi, Hamidreza [VerfasserIn] |
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| | van Straaten, Monique [VerfasserIn] |
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| | Zeelen, Johan P. [VerfasserIn] |
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| | Kelemen, Yosip [VerfasserIn] |
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| | Baehr, Carly [VerfasserIn] |
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| | Marker, Cheryl L. [VerfasserIn] |
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| | Ruf, Sandra [VerfasserIn] |
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| | Svirina, Anna [VerfasserIn] |
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| | Chandra, Monica [VerfasserIn] |
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| | Urban, Katharina [VerfasserIn] |
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| | Gkeka, Anastasia [VerfasserIn] |
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| | Kruse, Sebastian [VerfasserIn] |
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| | Baumann, Andreas [VerfasserIn] |
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| | Miller, Aubry K. [VerfasserIn] |
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| | Bartel, Marc [VerfasserIn] |
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| | Pravetoni, Marco [VerfasserIn] |
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| | Stebbins, Erec [VerfasserIn] |
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| | Papavasiliou, Nina [VerfasserIn] |
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| | Verdi, Joseph P. [VerfasserIn] |
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| Titel: | A trypanosome-derived immunotherapeutics platform elicits potent high-affinity antibodies, negating the effects of the synthetic opioid fentanyl |
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| Verf.angabe: | Gianna Triller, Evi P. Vlachou, Hamidreza Hashemi, Monique van Straaten, Johan P. Zeelen, Yosip Kelemen, Carly Baehr, Cheryl L. Marker, Sandra Ruf, Anna Svirina, Monica Chandra, Katharina Urban, Anastasia Gkeka, Sebastian Kruse, Andreas Baumann, Aubry K. Miller, Marc Bartel, Marco Pravetoni, C. Erec Stebbins, F. Nina Papavasiliou, and Joseph P. Verdi |
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| E-Jahr: | 2023 |
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| Jahr: | 30 January 2023 |
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| Umfang: | 28 S. |
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| Fussnoten: | Gesehen am 21.03.2023 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 42(2023), 2 vom: Jan., Artikel-ID 112049, Seite 1-28 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Poorly immunogenic small molecules pose challenges for the production of clinically efficacious vaccines and antibodies. To address this, we generate an immunization platform derived from the immunogenic surface coat of the African trypanosome. Through sortase-based conjugation of the target molecules to the variant surface glycoprotein (VSG) of the trypanosome surface coat, we develop VSG-immunogen array by sortase tagging (VAST). VAST elicits antigen-specific memory B cells and antibodies in a murine model after deploying the poorly immunogenic molecule fentanyl as a proof of concept. We also develop a single-cell RNA sequencing (RNA-seq)-based computational method that synergizes with VAST to specifically identify memory B cell-encoded antibodies. All computationally selected antibodies bind to fentanyl with picomolar affinity. Moreover, these antibodies protect mice from fentanyl effects after passive immunization, demonstrating the ability of these two coupled technologies to elicit therapeutic antibodies to challenging immunogens. |
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| DOI: | doi:10.1016/j.celrep.2023.112049 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.celrep.2023.112049 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2211124723000608 |
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| | DOI: https://doi.org/10.1016/j.celrep.2023.112049 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | antibody repertoire analysis |
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| | fentanyl |
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| | humoral immunity |
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| | immunological memory |
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| | opioid overdose |
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| | sortase |
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| | variant surface glycoprotein |
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| K10plus-PPN: | 1839678259 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ trypanosome-derived immunotherapeutics platform elicits potent high-affinity antibodies, negating the effects of the synthetic opioid fentanyl / Triller, Gianna [VerfasserIn]; 30 January 2023 (Online-Ressource)
