Yeast pre-rRNA processing and modification occur cotranscriptionally

• Verfasst von: Koš, Martin [VerfasserIn]
• Verfasst von: Tollervey, David [VerfasserIn]
• Titel: Yeast pre-rRNA processing and modification occur cotranscriptionally
• Verf.angabe: Martin Koš and David Tollervey
• E-Jahr: 2010
• Jahr: March 25, 2010
• Umfang: 12 S.
• Fussnoten: Gesehen am 2.03.2023
• Titel Quelle: Enthalten in: Molecular cell
• Ort Quelle: [Cambridge, Mass.] : Cell Press, 1997
• Jahr Quelle: 2010
• Band/Heft Quelle: 37(2010), 6 vom: März, Seite 809-820
• ISSN Quelle: 1097-4164
• DOI: doi:10.1016/j.molcel.2010.02.024
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: DNA
• Sach-SW: RNA
• Sach-SW: SYSBIO
• K10plus-PPN: 1839782684

Abstract

To better understand yeast ribosome synthesis, we developed techniques for the rapid harvesting and analysis of metabolically labeled cultures. Modeling of the resulting kinetic data allowed predicted lifetimes and processing patterns to be compared with the experimental data. This supported a transcription time for the 35S primary transcripts of ∼170 s at 30°C (∼40 nt s−1), with a high fraction (∼70%) of nascent transcripts cleaved at the early processing sites that generate the 20S precursor to the 18S rRNA. This level of nascent transcript cleavage apparently conflicted with previous reports that modification of yeast pre-rRNA exclusively occurred on released transcripts. A second round of high-resolution kinetic labeling showed that 20S pre-rRNA predominately undergoes methylation as nascent transcripts, whereas the 27S precursor to the 25S/5.8S rRNAs was partially methylated on the nascent transcript. The results demonstrate that quantitative analyses of pre-rRNA processing can yield important biological insights.