Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met) polymorphism

• Verfasst von: Horschitz, Sandra [VerfasserIn]
• Verfasst von: Frank, Josef [VerfasserIn]
• Verfasst von: Koch, Philipp [VerfasserIn]
• Titel: Generation of three induced pluripotent stem cell lines carrying different variants of the BDNF (Val66Met) polymorphism
• Verf.angabe: Sandra Horschitz, Josef Frank, Philipp Koch
• E-Jahr: 2023
• Jahr: February 2023
• Umfang: 4 S.
• Fussnoten: Online verfügbar 5 Dezember 2022 ; Gesehen am 28.03.2023
• Titel Quelle: Enthalten in: Stem cell research
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 2007
• Jahr Quelle: 2023
• Band/Heft Quelle: 66(2023) vom: Feb., Artikel-ID 102989, Seite 1-4
• ISSN Quelle: 1876-7753
• DOI: doi:10.1016/j.scr.2022.102989
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1840307544

Abstract

Brain-derived neurotrophic factor (BDNF) has been implicated in a multitude of neurodevelopmental processes including neuronal differentiation, axonal outgrowth, synaptic plasticity, or survival. One human-specific single nucleotide polymorphism (rs6265) in the BDNF gene causes a substitution of valine (Val) to methionine (Met) at codon 66 in the pro domain of the protein (Val66Met). This substitution is associated to reduced hippocampal volumes, poor performance on hippocampal-dependent memory tasks, and some mental disorders such as schizophrenia, depression or Alzheimer’s disease. Here we generated three iPSC lines from healthy donors, either homozygous (Val/Val and Met/Met) or heterozygous (Val/Met) for the polymorphism.