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Status: Bibliographieeintrag

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Verfasst von:Yee, Michelle [VerfasserIn]   i
 Walther, Tobias [VerfasserIn]   i
 Frischknecht, Friedrich [VerfasserIn]   i
 Douglas, Ross G. [VerfasserIn]   i
Titel:Divergent Plasmodium actin residues are essential for filament localization, mosquito salivary gland invasion and malaria transmission
Verf.angabe:Michelle Yee, Tobias Walther, Friedrich Frischknecht, Ross G. Douglas
E-Jahr:2022
Jahr:August 23, 2022
Umfang:27 S.
Fussnoten:Gesehen am 29.03.2023
Titel Quelle:Enthalten in: Public Library of SciencePLoS pathogens
Ort Quelle:Lawrence, Kan. : PLoS, 2005
Jahr Quelle:2022
Band/Heft Quelle:18(2022), 8 vom: Aug., Artikel-ID e1010779, Seite 1-27
ISSN Quelle:1553-7374
Abstract:Actin is one of the most conserved and ubiquitous proteins in eukaryotes. Its sequence has been highly conserved for its monomers to self-assemble into filaments that mediate essential cell functions such as trafficking, cell shape and motility. The malaria-causing parasite, Plasmodium, expresses a highly sequence divergent actin that is critical for its rapid motility at different stages within its mammalian and mosquito hosts. Each of Plasmodium actin’s four subdomains have divergent regions compared to canonical vertebrate actins. We previously identified subdomains 2 and 3 as providing critical contributions for parasite actin function as these regions could not be replaced by subdomains of vertebrate actins. Here we probed the contributions of individual divergent amino acid residues in these subdomains on parasite motility and progression. Non-lethal changes in these subdomains did not affect parasite development in the mammalian host but strongly affected progression through the mosquito with striking differences in transmission to and through the insect. Live visualization of actin filaments showed that divergent amino acid residues in subdomains 2 and 4 enhanced localization associated with filaments, while those in subdomain 3 negatively affected actin filaments. This suggests that finely tuned actin dynamics are essential for efficient organ entry in the mosquito vector affecting malaria transmission. This work provides residue level insight on the fundamental requirements of actin in highly motile cells.
DOI:doi:10.1371/journal.ppat.1010779
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1371/journal.ppat.1010779
 Volltext: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1010779
 DOI: https://doi.org/10.1371/journal.ppat.1010779
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Actin filaments
 Actins
 Dynamic actin filaments
 Parasitic diseases
 Parasitic life cycles
 Plasmodium
 Salivary glands
 Sporozoites
K10plus-PPN:1840463066
Verknüpfungen:→ Zeitschrift

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