Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa

• Verfasst von: Szőke, Anita [VerfasserIn]
• Verfasst von: Sárkány, Orsolya [VerfasserIn]
• Verfasst von: Schermann, Géza [VerfasserIn]
• Verfasst von: Kapuy, Orsolya [VerfasserIn]
• Verfasst von: Diernfellner, Axel [VerfasserIn]
• Verfasst von: Brunner, Michael [VerfasserIn]
• Verfasst von: Gyöngyösi, Norbert [VerfasserIn]
• Verfasst von: Káldi, Krisztina [VerfasserIn]
• Titel: Adaptation to glucose starvation is associated with molecular reorganization of the circadian clock in Neurospora crassa
• Verf.angabe: Anita Szőke, Orsolya Sárkány, Géza Schermann, Orsolya Kapuy, Axel CR Diernfellner, Michael Brunner, Norbert Gyöngyösi, Krisztina Káldi
• E-Jahr: 2023
• Jahr: 10 January 2023
• Umfang: 26 S.
• Fussnoten: Gesehen am 31.03.2023
• Titel Quelle: Enthalten in: eLife
• Ort Quelle: Cambridge : eLife Sciences Publications, 2012
• Jahr Quelle: 2023
• Band/Heft Quelle: 12(2023), Artikel-ID e79765, Seite 1-26
• ISSN Quelle: 2050-084X
• DOI: doi:10.7554/eLife.79765
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: circadian rhythm
• Sach-SW: glucose starvation
• Sach-SW: protein kinase A
• Sach-SW: protein phosphatase 2
• Sach-SW: transcriptome
• Sach-SW: white collar complex
• K10plus-PPN: 1840915412

Abstract

The circadian clock governs rhythmic cellular functions by driving the expression of a substantial fraction of the genome and thereby significantly contributes to the adaptation to changing environmental conditions. Using the circadian model organism Neurospora crassa, we show that molecular timekeeping is robust even under severe limitation of carbon sources, however, stoichiometry, phosphorylation and subcellular distribution of the key clock components display drastic alterations. Protein kinase A, protein phosphatase 2 A and glycogen synthase kinase are involved in the molecular reorganization of the clock. RNA-seq analysis reveals that the transcriptomic response of metabolism to starvation is highly dependent on the positive clock component WC-1. Moreover, our molecular and phenotypic data indicate that a functional clock facilitates recovery from starvation. We suggest that the molecular clock is a flexible network that allows the organism to maintain rhythmic physiology and preserve fitness even under long-term nutritional stress.