Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS

• Verfasst von: Gnann, Heike [VerfasserIn]
• Verfasst von: Engelmann, C. [VerfasserIn]
• Verfasst von: Skopp, Gisela [VerfasserIn]
• Verfasst von: Winkler, M. [VerfasserIn]
• Verfasst von: Auwärter, V. [VerfasserIn]
• Verfasst von: Dresen, S. [VerfasserIn]
• Verfasst von: Ferreirós, N. [VerfasserIn]
• Verfasst von: Wurst, F. M. [VerfasserIn]
• Verfasst von: Weinmann, W. [VerfasserIn]
• Titel: Identification of 48 homologues of phosphatidylethanol in blood by LC-ESI-MS/MS
• Verf.angabe: H. Gnann, C. Engelmann, G. Skopp, M. Winkler, V. Auwärter, S. Dresen, N. Ferreirós, F.M. Wurst, W. Weinmann
• E-Jahr: 2010
• Jahr: 2 February 2010
• Umfang: 9 S.
• Fussnoten: Gesehen am 05.04.2023
• Titel Quelle: Enthalten in: Analytical and bioanalytical chemistry
• Ort Quelle: Berlin : Springer, 2002
• Jahr Quelle: 2010
• Band/Heft Quelle: 396(2010), 7, Seite 2415-2423
• ISSN Quelle: 1618-2650
• DOI: doi:10.1007/s00216-010-3458-5
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Alcohol
• Sach-SW: Biomarker
• Sach-SW: Homologues
• Sach-SW: LC-MS/MS
• Sach-SW: Phosphatidylethanol
• Sach-SW: Phospholipids
• K10plus-PPN: 1841260061

Abstract

Phosphatidylethanol (PEth) is an abnormal phospholipid carrying two fatty acid chains. It is only formed in the presence of ethanol via the action of phospholipase D (PLD). Its use as a biomarker for alcohol consumption is currently under investigation. Previous methods for the analysis of PEth included high-performance liquid chromatography (HPLC) coupled to an evaporative light scattering detector (ELSD), which is unspecific for the different homologues—improved methods are now based on time of flight mass spectrometry (TOF-MS) and tandem mass spectrometry (MS/MS). The intention of this work was to identify as many homologues of PEth as possible. A screening procedure using multiple-reaction monitoring (MRM) for the identified homologues has subsequently been established. For our investigations, autopsy blood samples collected from heavy drinkers were used. Phosphatidylpropanol 16:0/18:1 (internal standard) was added to the blood samples prior to liquid-liquid extraction using borate buffer (pH 9), 2-propanol and n-hexane. After evaporation, the samples were redissolved in the mobile phase and injected into the LC-MS/MS system. Compounds were separated on a Luna Phenyl Hexyl column (50 mm × 2 mm, 3 µm) by gradient elution, using 2 mM ammonium acetate and methanol/acetone (95/5; v/v). A total of 48 homologues of PEth could be identified by using precursor ion and enhanced product ion scans (EPI).