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Verfasst von:Gobert, Vanessa [VerfasserIn]   i
 Osman, Dani [VerfasserIn]   i
 Bras, Stéphanie [VerfasserIn]   i
 Augé, Benoit [VerfasserIn]   i
 Boube, Muriel [VerfasserIn]   i
 Bourbon, Henri-Marc [VerfasserIn]   i
 Horn, Thomas [VerfasserIn]   i
 Boutros, Michael [VerfasserIn]   i
 Haenlin, Marc [VerfasserIn]   i
 Waltzer, Lucas [VerfasserIn]   i
Titel:A genome-wide RNA interference screen identifies a differential role of the Mediator CDK8 module subunits for GATA/ RUNX-activated transcription in Drosophila
Verf.angabe:Vanessa Gobert, Dani Osman, Stéphanie Bras, Benoit Augé, Muriel Boube, Henri-Marc Bourbon, Thomas Horn, Michael Boutros, Marc Haenlin, and Lucas Waltzer
E-Jahr:2010
Jahr:Juni 2010
Umfang:12 S.
Fussnoten:Gesehen am 05.04.2023
Titel Quelle:Enthalten in: Molecular and cellular biology
Ort Quelle:Washington, DC : American Society for Microbiology, 1981
Jahr Quelle:2010
Band/Heft Quelle:30(2010), 11, Seite 2837-2848
ISSN Quelle:0270-7306
Abstract:Transcription factors of the RUNX and GATA families play key roles in the control of cell fate choice and differentiation, notably in the hematopoietic system. During Drosophila hematopoiesis, the RUNX factor Lozenge and the GATA factor Serpent cooperate to induce crystal cell differentiation. We used Serpent/Lozenge-activated transcription as a paradigm to identify modulators of GATA/RUNX activity by a genome-wide RNA interference screen in cultured Drosophila blood cells. Among the 129 factors identified, several belong to the Mediator complex. Mediator is organized in three modules plus a regulatory “CDK8 module,” composed of Med12, Med13, CycC, and Cdk8, which has long been thought to behave as a single functional entity. Interestingly, our data demonstrate that Med12 and Med13 but not CycC or Cdk8 are essential for Serpent/Lozenge-induced transactivation in cell culture. Furthermore, our in vivo analysis of crystal cell development show that, while the four CDK8 module subunits control the emergence and the proliferation of this lineage, only Med12 and Med13 regulate its differentiation. We thus propose that Med12/Med13 acts as a coactivator for Serpent/Lozenge during crystal cell differentiation independently of CycC/Cdk8. More generally, we suggest that the set of conserved factors identified herein may regulate GATA/RUNX activity in mammals.
DOI:doi:10.1128/MCB.01625-09
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1128/MCB.01625-09
 Volltext: https://journals.asm.org/doi/10.1128/MCB.01625-09
 DOI: https://doi.org/10.1128/MCB.01625-09
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1841615803
Verknüpfungen:→ Zeitschrift

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