| |  Online-Ressource |
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| Verfasst von: | Hegge, Stephan [VerfasserIn]  |
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| | Kudryashev, Mikhail [VerfasserIn] |
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| | Barniol, Luis Fernando [VerfasserIn] |
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| | Frischknecht, Friedrich [VerfasserIn] |
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| Titel: | Key factors regulating Plasmodium berghei sporozoite survival and transformation revealed by an automated visual assay |
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| Verf.angabe: | Stephan Hegge, Mikhail Kudryashev, Luis Barniol, Friedrich Frischknecht |
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| E-Jahr: | 2010 |
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| Jahr: | 26 August 2010 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 11.04.2023 |
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| Titel Quelle: | Enthalten in: Federation of American Societies for Experimental BiologyThe FASEB journal |
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| Ort Quelle: | Hoboken, NJ : Wiley, 1987 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 24(2010), 12 vom: Aug., Seite 5003-5012 |
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| ISSN Quelle: | 1530-6860 |
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| Abstract: | Malaria is transmitted to the host when Plasmodium sporozoites are injected by a mosquito vector. Sporozoites eventually enter hepatocytes, where they differentiate into liver-stage parasites. During the first hours after hepatocyte invasion, the crescent-shaped sporozoites transform into spherical intracellular exoerythrocytic parasites. This process, which precedes genome replication, can be mimicked in vitro in the absence of host cells. Here, we developed an automated method to follow transformation and cell death of sporozoites in vitro. This assay provides a rapid tool to test sporozoite survival and to screen for antiparasitic drugs. We found that extracellular bicarbonate and high temperature trigger transformation, whereas physiological serum albumin concentrations and media lacking bicarbonate delayed sporozoite death. Because bicarbonate also triggers ookinete transformation and exflagellation of gametocytes, we suggest that a common molecular mechanism regulates similar aspects of stage conversion in Plasmodium.—Hegge, S., Kudryashev, M., Barniol, L., Frischknecht, F. Key factors regulating Plasmodium berghei sporozoite survival and transformation revealed by an automated visual assay. FASEB J. 24, 5003-5012 (2010). www.fasebj.org |
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| DOI: | doi:10.1096/fj.10.164814 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1096/fj.10.164814 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1096/fj.10.164814 |
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| | DOI: https://doi.org/10.1096/fj.10.164814 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Apicomplexa |
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| | bicarbonate |
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| | differentiation |
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| | glucose |
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| | screening |
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| K10plus-PPN: | 1842004883 |
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| Verknüpfungen: | → Zeitschrift |
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Key factors regulating Plasmodium berghei sporozoite survival and transformation revealed by an automated visual assay / Hegge, Stephan [VerfasserIn]; 26 August 2010 (Online-Ressource)
