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| Verfasst von: | Horn, Patrick [VerfasserIn]  |
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| | Bokermann, Gudrun [VerfasserIn] |
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| | Cholewa, Dominik [VerfasserIn] |
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| | Bork, Simone [VerfasserIn] |
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| | Walenda, Thomas [VerfasserIn] |
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| | Koch, Carmen [VerfasserIn] |
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| | Drescher, Wolf [VerfasserIn] |
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| | Hutschenreuther, Gabriele [VerfasserIn] |
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| | Zenke, Martin [VerfasserIn] |
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| | Ho, Anthony Dick [VerfasserIn] |
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| | Wagner, Wolfgang [VerfasserIn] |
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| Titel: | Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells |
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| Verf.angabe: | Patrick Horn, Gudrun Bokermann, Dominik Cholewa, Simone Bork, Thomas Walenda, Carmen Koch, Wolf Drescher, Gabriele Hutschenreuther, Martin Zenke, Anthony D. Ho & Wolfgang Wagner |
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| E-Jahr: | 2010 |
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| Jahr: | [November 2010] |
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| Umfang: | 11 S. |
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| Illustrationen: | Illustrationen, Diagramme |
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| Fussnoten: | Gesehen am 17.04.2023 |
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| Titel Quelle: | Enthalten in: Cytotherapy |
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| Ort Quelle: | Abingdon : Taylor & Francis Group, 1999 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 12(2010), 7 vom: Nov., Seite 888-898 |
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| ISSN Quelle: | 1477-2566 |
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| Abstract: | Background aims - Culture medium for mesenchymal stromal cells (MSC) is frequently supplemented with fetal calf serum (FCS). FCS can induce xenogeneic immune reactions, transmit bovine pathogens and has a high lot-to-lot variability that hampers reproducibility of results. Several studies have demonstrated that pooled human platelet lysate (HPL) provides an attractive alternative for FCS. However, little is known about the variation between different platelet lysates. - Methods - We compared activities of individual HPL on initial fibroblastoid colony-forming units (CFU-F), proliferation, in vitro differentiation and long-term culture. These data were correlated with chemokine profiles of HPL. - Results - Isolation of MSC with either HPL or FCS resulted in similar CFU-F frequency, colony morphology, immunophenotype and adipogenic differentiation potential. Osteogenic differentiation was even more pronounced in HPL than FCS. There were significant differences in MSC proliferation with different HPL, but it was always higher in comparison with FCS. Cell growth correlated with the concentration of platelet-derived growth factor (PDGF) and there was a moderate association with platelet counts. All HPL facilitated expansion for more than 20 population doublings. - Conclusions - Taken together, reliable long-term expansion was possible with all HPL, although there was some variation in platelet lysates of individual units. Therefore the use of donor recipient-matched or autologous HPL is feasible for therapeutic MSC products. |
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| DOI: | doi:10.3109/14653249.2010.501788 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3109/14653249.2010.501788 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1465324910704564 |
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| | DOI: https://doi.org/10.3109/14653249.2010.501788 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | colony-forming units |
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| | cytokine profile |
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| | fetal calf serum |
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| | long-term culture |
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| | mesenchymal stromal cells |
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| | platelet lysate |
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| K10plus-PPN: | 1842962418 |
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| Verknüpfungen: | → Zeitschrift |
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Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells / Horn, Patrick [VerfasserIn]; [November 2010] (Online-Ressource)
