Design and evaluation of genome-wide libraries for RNA interference screens

• Verfasst von: Horn, Thomas [VerfasserIn]
• Verfasst von: Sandmann, Thomas [VerfasserIn]
• Verfasst von: Boutros, Michael [VerfasserIn]
• Titel: Design and evaluation of genome-wide libraries for RNA interference screens
• Verf.angabe: Thomas Horn, Thomas Sandmann and Michael Boutros
• E-Jahr: 2010
• Jahr: 15 June 2010
• Umfang: 12 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 17.04.2023
• Titel Quelle: Enthalten in: Genome biology
• Ort Quelle: London : BioMed Central, 2000
• Jahr Quelle: 2010
• Band/Heft Quelle: 11(2010), 6 vom: Juni, Artikel-ID R61, Seite 1-12
• ISSN Quelle: 1474-760X
• DOI: doi:10.1186/gb-2010-11-6-r61
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Efficiency Score
• Sach-SW: Independent Design
• Sach-SW: RNAi Reagent
• Sach-SW: RNAi Screen
• Sach-SW: Seed Match
• K10plus-PPN: 1842964313

Abstract

RNA interference (RNAi) screens have enabled the systematic analysis of many biological processes in cultured cells and whole organisms. The success of such screens and the interpretation of the data depend on the stringent design of RNAi libraries. We describe and validate NEXT-RNAi, a software for the automated design and evaluation of RNAi sequences on a genome-wide scale. NEXT-RNAi is implemented as open-source software and is accessible at http://www.nextrnai.org/.