Generation of neuronal cells from human peripheral blood mononuclear cells

• Verfasst von: Horschitz, Sandra [VerfasserIn]
• Verfasst von: Meyer-Lindenberg, Andreas [VerfasserIn]
• Verfasst von: Schloss, Patrick [VerfasserIn]
• Titel: Generation of neuronal cells from human peripheral blood mononuclear cells
• Verf.angabe: Sandra Horschitz, Andreas Meyer-Lindenberg, and Patrick Schloss
• E-Jahr: 2010
• Jahr: [2010]
• Umfang: 6 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 17.04.2023
• Titel Quelle: Enthalten in: Neuroreport
• Ort Quelle: London : Lippincott Williams & Wilkins, 1990
• Jahr Quelle: 2010
• Band/Heft Quelle: 21(2010), 3, Seite 185-190
• ISSN Quelle: 1473-558X
• DOI: doi:10.1097/WNR.0b013e328334be4e
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1842966251

Abstract

We have examined the potency of two methods for the neuronal differentiation of embryonic stem cells on the generation of neuronal cells from human blood cells. A mixture of mononuclear cells from peripheral blood cells expressing monocytic, hematopoietic, and mesenchymal cell surface markers were exposed to all-trans retinoic acid, epidermal growth factor, and basic fibroblast growth factor (method A), or epidermal growth factor, fibroblast growth factor 8b, sonic hedgehog and ascorbic acid (method B). Both methods led to the generation of neuronal cells as judged by changes in morphology and the expression of the neuronal markers microtubule-associated protein type 2, tau, and β-tubulin III. Differentiation according to method B favoured the development of neurons also expressing the dopamine transporter.