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| Verfasst von: | Grimm, Elisabeth [VerfasserIn]  |
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| | Hoeven, Franciscus van der [VerfasserIn] |
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| | Sardella, Donato [VerfasserIn] |
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| | Willig, Katrin I. [VerfasserIn] |
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| | Engel, Ulrike [VerfasserIn] |
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| | Veits, Nisha [VerfasserIn] |
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| | Engel, Robert [VerfasserIn] |
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| | Cavalcanti-Adam, Elisabetta A. [VerfasserIn] |
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| | Bestvater, Felix [VerfasserIn] |
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| | Bordoni, Luca [VerfasserIn] |
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| | Jennemann, Richard [VerfasserIn] |
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| | Schönig, Kai [VerfasserIn] |
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| | Schiessl, Ina Maria [VerfasserIn] |
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| | Sandhoff, Roger [VerfasserIn] |
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| Titel: | A clathrin light chain |
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| Titelzusatz: | a reporter mouse for in vivo imaging of endocytosis |
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| Verf.angabe: | Elisabeth Grimm, Franciscus van der Hoeven, Donato Sardella, Katrin I. Willig, Ulrike Engel, Nisha Veits, Robert Engel, Elisabetta Ada Cavalcanti-Adam, Felix Bestvater, Luca Bordoni, Richard Jennemann, Kai Schönig, Ina Maria Schiessl, Roger Sandhoff |
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| E-Jahr: | 2022 |
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| Jahr: | September 23, 2022 |
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| Umfang: | 24 S. |
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| Fussnoten: | Gesehen am 19.04.2023 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 17(2022), 9, Artikel-ID e0273660, Seite 1-24 |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | Clathrin-mediated endocytosis (CME) is one of the best studied cellular uptake pathways and its contributions to nutrient uptake, receptor signaling, and maintenance of the lipid membrane homeostasis have been already elucidated. Today, we still have a lack of understanding how the different components of this pathway cooperate dynamically in vivo. Therefore, we generated a reporter mouse model for CME by fusing eGFP endogenously in frame to clathrin light chain a (Clta) to track endocytosis in living mice. The fusion protein is expressed in all tissues, but in a cell specific manner, and can be visualized using fluorescence microscopy. Recruitment to nanobeads recorded by TIRF microscopy validated the functionality of the Clta-eGFP reporter. With this reporter model we were able to track the dynamics of Alexa594-BSA uptake in kidneys of anesthetized mice using intravital 2-photon microscopy. This reporter mouse model is not only a suitable and powerful tool to track CME in vivo in genetic or disease mouse models it can also help to shed light into the differential roles of the two clathrin light chain isoforms in health and disease. |
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| DOI: | doi:10.1371/journal.pone.0273660 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1371/journal.pone.0273660 |
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| | Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0273660 |
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| | DOI: https://doi.org/10.1371/journal.pone.0273660 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Albumins |
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| | Cell membranes |
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| | Endocytosis |
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| | In vivo imaging |
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| | Kidneys |
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| | Membrane proteins |
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| | Mouse models |
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| | Small intestine |
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| K10plus-PPN: | 1843125056 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ clathrin light chain / Grimm, Elisabeth [VerfasserIn]; September 23, 2022 (Online-Ressource)
