Navigation überspringen
Universitätsbibliothek Heidelberg
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Oeing, Christian [VerfasserIn]   i
 Pepin, Mark [VerfasserIn]   i
 Saul, Kerstin B. [VerfasserIn]   i
 Seyhan Agircan, Ayca [VerfasserIn]   i
 Assenov, Yassen [VerfasserIn]   i
 Merkel, Tobias S. [VerfasserIn]   i
 Sedaghat-Hamedani, Farbod [VerfasserIn]   i
 Weis, Tanja [VerfasserIn]   i
 Meder, Benjamin [VerfasserIn]   i
 Guan, Kaomei [VerfasserIn]   i
 Plass, Christoph [VerfasserIn]   i
 Weichenhan, Dieter [VerfasserIn]   i
 Siede, Dominik [VerfasserIn]   i
 Backs, Johannes [VerfasserIn]   i
Titel:Indirect epigenetic testing identifies a diagnostic signature of cardiomyocyte DNA methylation in heart failure
Verf.angabe:Christian U. Oeing, Mark E. Pepin, Kerstin B. Saul, Ayça Seyhan Agircan, Yassen Assenov, Tobias S. Merkel, Farbod Sedaghat-Hamedani, Tanja Weis, Benjamin Meder, Kaomei Guan, Christoph Plass, Dieter Weichenhan, Dominik Siede, Johannes Backs
E-Jahr:2023
Jahr:20 March 2023
Umfang:16 S.
Fussnoten:Gesehen am 25.04.2023
Titel Quelle:Enthalten in: Basic research in cardiology
Ort Quelle:[Darmstadt u.a.] : Steinkopff, 1937
Jahr Quelle:2023
Band/Heft Quelle:118(2023) vom: März, Artikel-ID 9, Seite 1-16
ISSN Quelle:1435-1803
Abstract:Precision-based molecular phenotyping of heart failure must overcome limited access to cardiac tissue. Although epigenetic alterations have been found to underlie pathological cardiac gene dysregulation, the clinical utility of myocardial epigenomics remains narrow owing to limited clinical access to tissue. Therefore, the current study determined whether patient plasma confers indirect phenotypic, transcriptional, and/or epigenetic alterations to ex vivo cardiomyocytes to mirror the failing human myocardium. Neonatal rat ventricular myocytes (NRVMs) and single-origin human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and were treated with blood plasma samples from patients with dilated cardiomyopathy (DCM) and donor subjects lacking history of cardiovascular disease. Following plasma treatments, NRVMs and hiPSC-CMs underwent significant hypertrophy relative to non-failing controls, as determined via automated high-content screening. Array-based DNA methylation analysis of plasma-treated hiPSC-CMs and cardiac biopsies uncovered robust, and conserved, alterations in cardiac DNA methylation, from which 100 sites were validated using an independent cohort. Among the CpG sites identified, hypo-methylation of the ATG promoter was identified as a diagnostic marker of HF, wherein cg03800765 methylation (AUC = 0.986, P < 0.0001) was found to out-perform circulating NT-proBNP levels in differentiating heart failure. Taken together, these findings support a novel approach of indirect epigenetic testing in human HF.
DOI:doi:10.1007/s00395-022-00954-3
URL:kostenfrei: Volltext: https://doi.org/10.1007/s00395-022-00954-3
 DOI: https://doi.org/10.1007/s00395-022-00954-3
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:DNA methylation
 Epigenetics
 Heart failure
 Pilot study
 Precision medicine
K10plus-PPN:1843508974
Verknüpfungen:→ Zeitschrift
 
 
Lokale URL UB: Zum Volltext

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/69068885   QR-Code
zum Seitenanfang