Elucidating anomalous protein diffusion in living cells with fluorescence correlation spectroscopy

• Verfasst von: Malchus, Nina [VerfasserIn]
• Verfasst von: Weiß, Matthias [VerfasserIn]
• Titel: Elucidating anomalous protein diffusion in living cells with fluorescence correlation spectroscopy
• Titelzusatz: facts and pitfalls
• Verf.angabe: Nina Malchus, Matthias Weiss
• Jahr: 2010
• Umfang: 8 S.
• Fussnoten: Online veröffentlicht am 7. Juli 2009 ; Gesehen am 02.05.2023
• Titel Quelle: Enthalten in: Journal of fluorescence
• Ort Quelle: New York, NY : Springer Science + Business Media B.V., 1991
• Jahr Quelle: 2010
• Band/Heft Quelle: 20(2010), 1, Seite 19-26
• ISSN Quelle: 1573-4994
• DOI: doi:10.1007/s10895-009-0517-4
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Anomalous diffusion
• Sach-SW: Fluorescence correlation spectroscopy
• Sach-SW: Macromolecular crowding
• Sach-SW: Membranes
• K10plus-PPN: 1844113256

Abstract

Anomalous protein diffusion has been frequently observed in intracellular fluids and on membranes of living cells. Indeed, a large variety of specimen, from bacteriae to mammalian cells, and several non-invasive measurement techniques, e.g. fluorescence correlation spectroscopy, have revealed that the mean square displacement (MSD) of proteins in vivo is often characterized by an anomalous power-law increase ⟨r(t)2⟩∼tα with 0.5 < α ≤ 0.8. Here, we review these results with a particular focus on fluorescence correlation spectroscopy, and we report on possible causes of variations of the anomaly degree α. Moreover, we highlight generic consequences of anomalous diffusion that are likely to play an important role in the cellular context.