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| Verfasst von: | Ma, Mingming [VerfasserIn]  |
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| | Luo, Qiao [VerfasserIn] |
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| | Fan, Lijing [VerfasserIn] |
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| | Li, Weilong [VerfasserIn] |
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| | Li, Qiang [VerfasserIn] |
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| | Meng, Yu [VerfasserIn] |
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| | Yun, Chen [VerfasserIn] |
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| | Wu, Hongwei [VerfasserIn] |
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| | Lu, Yongping [VerfasserIn] |
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| | Cui, Shuang [VerfasserIn] |
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| | Liu, Fanna [VerfasserIn] |
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| | Hu, Bo [VerfasserIn] |
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| | Guan, Baozhang [VerfasserIn] |
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| | Liu, Huanhuan [VerfasserIn] |
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| | Huang, Shengling [VerfasserIn] |
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| | Liang, Wenxue [VerfasserIn] |
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| | Morgera, Stanislao [VerfasserIn] |
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| | Krämer, Bernhard [VerfasserIn] |
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| | Luan, Shaodong [VerfasserIn] |
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| | Yin, Lianghong [VerfasserIn] |
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| | Hocher, Berthold [VerfasserIn] |
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| Titel: | The urinary exosomes derived from premature infants attenuate cisplatin-induced acute kidney injury in mice via microRNA-30a-5p/mitogen-activated protein kinase 8 (MAPK8) |
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| Verf.angabe: | Mingming Ma, Qiao Luo, Lijing Fan, Weilong Li, Qiang Li, Yu Meng, Chen Yun, Hongwei Wu, Yongping Lu, Shuang Cui, Fanna Liu, Bo Hu, Baozhang Guan, Huanhuan Liu, Shengling Huang, Wenxue Liang, Stanislao Morgera, Bernhard Krämer, Shaodong Luan, Lianghong Yin, and Berthold Hocher |
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| E-Jahr: | 2022 |
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| Jahr: | 08 Jan 2022 |
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| Umfang: | 6 S. |
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| Fussnoten: | Gesehen am 04.05.2023 |
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| Titel Quelle: | Enthalten in: Bioengineered |
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| Ort Quelle: | London : Taylor & Francis, 2012 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 13(2022), 1, Seite 1650-1665 |
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| ISSN Quelle: | 2165-5987 |
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| Abstract: | Acute kidney injury (AKI) is a susceptible factor for chronic kidney disease (CKD). There is still a lack of effective prevention methods in clinical practice. This study investigated the protective effect of the urinary exosomes from premature infants on cisplatin-induced acute kidney injury. Here we isolated exosomes from the fresh urine of premature infants. A C57BL/6 mice model of cisplatin-induced acute kidney injury was given 100 ug urinary exosomes 24 hours after model establishment. The kidneys were collected for pathological examination and the evaluation of renal tubular damage and apoptosis. In the in vitro experiment, human renal cortex/proximal tubular cells (HK-2) were induced by cisplatin to assess the effect of the urine exosomes from premature infants. Exosome microRNA (miRNA) sequencing technology was applied to investigate the miRNAs enriched in exosomes and the dual-luciferase gene reporter system to examine the targeting relationship of the miRNA with target genes. The results indicated that the urinary exosomes could decrease the serum creatinine level and the apoptosis of renal tubular cells, and reduce mice mortality. In addition, miR-30a-5p was the most abundant miRNA in the exosomes. It protected HK-2 cells from cisplatin-induced apoptosis by targeting and down-regulating the mitogen-activated protein kinase 8 (MAPK8). Together, our findings identified that the urinary exosomes derived from premature infants alleviated cisplatin-induced acute kidney injury and inhibited the apoptosis of HK-2 via miR-30a-5p, which could target MAPK8. These findings implied that urinary exosomes from premature infants riched in miR-30a-5p might become a potential treatment for AKI. |
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| DOI: | doi:10.1080/21655979.2021.2021686 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1080/21655979.2021.2021686 |
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| | DOI: https://doi.org/10.1080/21655979.2021.2021686 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | acute kidney injury |
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| | microRNA-30a-5p |
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| | mitogen-activated protein kinase 8 |
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| | Urinary exosomes |
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| K10plus-PPN: | 1844593592 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ urinary exosomes derived from premature infants attenuate cisplatin-induced acute kidney injury in mice via microRNA-30a-5p/mitogen-activated protein kinase 8 (MAPK8) / Ma, Mingming [VerfasserIn]; 08 Jan 2022 (Online-Ressource)
