Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Podevin, Philippe [VerfasserIn]   i
 Carpentier, Arnaud [VerfasserIn]   i
 Pène, Véronique [VerfasserIn]   i
 Aoudjehane, Lynda [VerfasserIn]   i
 Carrière, Matthieu [VerfasserIn]   i
 Zaïdi, Sakina [VerfasserIn]   i
 Hernandez, Céline [VerfasserIn]   i
 Calle, Vanessa [VerfasserIn]   i
 Méritet, Jean-François [VerfasserIn]   i
 Scatton, Olivier [VerfasserIn]   i
 Dreux, Marlène [VerfasserIn]   i
 Cosset, François-Loïc [VerfasserIn]   i
 Wakita, Takaji [VerfasserIn]   i
 Bartenschlager, Ralf [VerfasserIn]   i
 Demignot, Sylvie [VerfasserIn]   i
 Conti, Filoména [VerfasserIn]   i
 Rosenberg, Arielle R. [VerfasserIn]   i
 Calmus, Yvon [VerfasserIn]   i
Titel:Production of infectious Hepatitis C virus in primary cultures of human adult hepatocytes
Verf.angabe:Philippe Podevin, Arnaud Carpentier, Véronique Pène, Lynda Aoudjehane, Matthieu Carrière, Sakina Zaïdi, Céline Hernandez, Vanessa Calle, Jean-François Méritet, Olivier Scatton, Marlène Dreux, François-Loïc Cosset, Takaji Wakita, Ralf Bartenschlager, Sylvie Demignot, Filoména Conti, Arielle R. Rosenberg, and Yvon Calmus
E-Jahr:2010
Jahr:1 July 2010
Umfang:16 S.
Fussnoten:Gesehen am 04.05.2023
Titel Quelle:Enthalten in: Gastroenterology
Ort Quelle:New York, NY : Elsevier, 1949
Jahr Quelle:2010
Band/Heft Quelle:139(2010), 4 vom: Juli, Seite 1355-1364.e6
ISSN Quelle:1528-0012
Abstract:Background & Aims - Although hepatitis C virus (HCV) can be grown in the hepatocarcinoma-derived cell line Huh-7, a cell-culture model is needed that supports its complete, productive infection cycle in normal, quiescent, highly differentiated human hepatocytes. We sought to develop such a system. - Methods - Primary cultures of human adult hepatocytes were inoculated with HCV derived from Huh-7 cell culture (HCVcc) and monitored for expression of hepatocyte differentiation markers and replication of HCV. Culture supernatants were assayed for HCV RNA, core antigen, and infectivity titer. The buoyant densities of input and progeny virus were compared in iodixanol gradients. - Results - While retaining expression of differentiation markers, primary hepatocytes supported the complete infectious cycle of HCV, including production of significant titers of new infectious progeny virus, which was called primary-culture-derived virus (HCVpc). Compared with HCVcc, HCVpc had lower average buoyant density and higher specific infectivity; this was similar to the characteristics of virus particles associated with the very-low-density lipoproteins that are produced during in vivo infection. These properties were lost after re-culture of HCVpc in poorly differentiated Huh-7 cells, suggesting that authentic virions can be produced only by normal hepatocytes that secrete authentic very-low-density lipoproteins. - Conclusions - We have established a cell-culture-based system that allows production of infectious HCV in physiologically relevant human hepatocytes. This provides a useful tool for the study of HCV interactions with its natural host cell and for the development of antiviral therapies.
DOI:doi:10.1053/j.gastro.2010.06.058
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1053/j.gastro.2010.06.058
 Volltext: https://www.sciencedirect.com/science/article/pii/S0016508510010012
 DOI: https://doi.org/10.1053/j.gastro.2010.06.058
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Antiviral Drug Assay
 Cell Permissiveness
 Hepatocellular Physiology
 Virus Propagation
K10plus-PPN:1844599477
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/69072107   QR-Code
zum Seitenanfang