Farnesylated nuclear proteins Kugelkern and Lamin Dm0 affect nuclear morphology by directly interacting with the nuclear membrane

• Verfasst von: Polychronidou, Maria [VerfasserIn]
• Verfasst von: Hellwig, Andrea [VerfasserIn]
• Verfasst von: Grosshans, Jörg [VerfasserIn]
• Titel: Farnesylated nuclear proteins Kugelkern and Lamin Dm0 affect nuclear morphology by directly interacting with the nuclear membrane
• Verf.angabe: Maria Polychronidou, Andrea Hellwig, and Jörg Grosshans
• E-Jahr: 2010
• Jahr: 4 Aug 2010
• Umfang: 12 S.
• Fussnoten: Gesehen am 04.05.2023
• Titel Quelle: Enthalten in: Molecular biology of the cell
• Ort Quelle: Bethesda, Md. : American Society for Cell Biology, 1992
• Jahr Quelle: 2010
• Band/Heft Quelle: 21(2010), 19 vom: Aug., Seite 3409-3420
• ISSN Quelle: 1939-4586
• DOI: doi:10.1091/mbc.e10-03-0230
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1844608670

Abstract

Nuclear shape changes are observed during a variety of developmental processes, pathological conditions, and ageing. The mechanisms underlying nuclear shape changes in the above-mentioned situations have mostly remained unclear. To address the molecular mechanism behind nuclear shape changes, we analyzed how the farnesylated nuclear envelope proteins Kugelkern and lamin Dm0 affect the structure of the nuclear membrane. We found that Kugelkern and lamin Dm0 affect nuclear shape without requiring filament formation or the presence of a classical nuclear lamina. We also could show that the two proteins do not depend on a group of selected inner nuclear membrane proteins for their localization to the nuclear envelope. Surprisingly, we found that farnesylated Kugelkern and lamin Dm0 protein constructs change the morphology of protein-free liposomes. Based on these findings, we propose that farnesylated proteins of the nuclear membrane induce nuclear shape changes by being asymmetrically inserted into the phospholipid bilayer via their farnesylated C-terminal part.