Impaired skin regeneration and remodeling after cutaneous injury and chemically induced hyperplasia in taps-transgenic mice

• Verfasst von: Hildenbrand, Maike [VerfasserIn]
• Verfasst von: Rhiemeier, Verena Margrit [VerfasserIn]
• Verfasst von: Hartenstein, Bettina [VerfasserIn]
• Verfasst von: Lahrmann, Bernd [VerfasserIn]
• Verfasst von: Grabe, Niels [VerfasserIn]
• Verfasst von: Angel, Peter [VerfasserIn]
• Verfasst von: Heß, Jochen [VerfasserIn]
• Titel: Impaired skin regeneration and remodeling after cutaneous injury and chemically induced hyperplasia in taps-transgenic mice
• Verf.angabe: Maike Hildenbrand, Verena Rhiemeier, Bettina Hartenstein, Bernd Lahrmann, Niels Grabe, Peter Angel and Jochen Hess
• E-Jahr: 2010
• Jahr: [July 2010]
• Umfang: 9 S.
• Fussnoten: Gesehen am 10.05.2023
• Titel Quelle: Enthalten in: The journal of investigative dermatology
• Ort Quelle: Amsterdam : Elsevier, 1938
• Jahr Quelle: 2010
• Band/Heft Quelle: 130(2010), 7 vom: Juli, Seite 1922-1930
• ISSN Quelle: 1523-1747
• DOI: doi:10.1038/jid.2010.54
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1844963616

Abstract

Recently, we identified an AP-1-dependent target gene in 12-O-tetradecanoylphorbol-13-acetate (TPA)-treated mouse back skin, which encodes a retroviral-like aspartic proteinase (Taps/Asprv1). Taps expression was detected almost exclusively in stratified epithelia of mouse embryos and adult tissues, and enhanced protein levels were present in several non-neoplastic human skin disorders, implicating a crucial role for differentiation and homeostasis of multilayered epithelia. Here, we generated a mouse model in which Taps transgene expression is under the control of the human ubiquitin C promoter (UBC-Taps). Although no obvious phenotype was observed in normal skin development and homeostasis, these mice showed a significant delay in cutaneous wound closure compared with control animals. Shortly after re-epithelialization, we found an increase in keratinocytes in the stratum granulosum, which express Filaggrin, a late differentiation marker. A hypergranulosum-like phenotype with increased numbers of Filaggrin-positive keratinocytes was also observed in UBC-Taps mice after administration of TPA. In summary, these data show that aberrant Taps expression causes impaired skin regeneration and skin remodeling after cutaneous injury and chemically induced hyperplasia.