Gap junctions between regulatory T cells and dendritic cells prevent sensitization of CD8+ T cells

• Verfasst von: Ring, Sabine [VerfasserIn]
• Verfasst von: Karakhanova, Svetlana [VerfasserIn]
• Verfasst von: Johnson, Theron S. [VerfasserIn]
• Verfasst von: Enk, Alexander [VerfasserIn]
• Verfasst von: Mahnke, Karsten [VerfasserIn]
• Titel: Gap junctions between regulatory T cells and dendritic cells prevent sensitization of CD8+ T cells
• Verf.angabe: Sabine Ring, PhD, Svetlana Karakhanova, PhD, Theron Johnson, PhD, Alexander H. Enk, MD, and Karsten Mahnke, PhD
• E-Jahr: 2010
• Jahr: 1 January 2010
• Umfang: 17 S.
• Fussnoten: Gesehen am 24.05.2023 ; Im Titel ist das "+"-Zeichen hochgestellt
• Titel Quelle: Enthalten in: The journal of allergy and clinical immunology
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 1971
• Jahr Quelle: 2010
• Band/Heft Quelle: 125(2010), 1 vom: Jan., Seite 237-246, e1-e7
• ISSN Quelle: 1097-6825
• DOI: doi:10.1016/j.jaci.2009.10.025
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Contact hypersensitivity
• Sach-SW: dendritic cells
• Sach-SW: gap junctions
• Sach-SW: regulatory T cells
• K10plus-PPN: 184610629X

Abstract

Background - Regulatory T (Treg) cells suppress the sensitization phase of experimental contact hypersensitivity (CHS) reactions when injected before hapten application. - Objective - Our aim was to analyze the mechanisms by which Treg cells suppress the sensitization phase of CHS reactions. - Methods - Treg cells were labeled with different fluorescent dyes and injected into naive mice directly before sensitization with the hapten 2,4,6-trinitro-1-chlorobenzene. Two days after sensitization, the lymphoid organs were analyzed for the presence of Treg cells and engagement of gap junctions with other cells. Dendritic cells (DCs) and effector CD8+T cells were isolated from the draining lymph nodes (LNs) of the differently treated groups, analyzed by using FACS for activation markers, and assessed for the T-cell stimulatory capacity of the DCs and the priming of effector T cells. - Results - Only the LN-homing Treg cells suppressed the sensitization phase in CHS reactions by means of establishing gap junctions with DCs in the dLNs. This gap junctional intercellular communication led to downregulation of T-cell costimulatory molecules on the surface of the DCs, abrogating the priming, activation, and proliferation of hapten-specific CD8+T cells. Consequently, the ear-swelling response induced by challenge with the respective hapten was prevented. - Conclusion - Treg cells not only modulate ongoing CD4+T cell-mediated immune reactions at tissue sites but also abrogate the de novo induction of CD8+T cell-driven immune reactions by interfering with T-cell stimulatory activity of DCs through gap junctional intercellular communication.