| |  Online-Ressource |
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| Verfasst von: | Xu, Ruiyao [VerfasserIn]  |
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| | Wang, Xianxian [VerfasserIn] |
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| | Safi, Sadia [VerfasserIn] |
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| | Braunegger, Nico [VerfasserIn] |
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| | Hipgrave Ederveen, Agnes [VerfasserIn] |
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| | Rottmann, Michelle [VerfasserIn] |
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| | Wittbrodt, Joachim [VerfasserIn] |
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| | Wuhrer, Manfred [VerfasserIn] |
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| | Wesslowski, Janine [VerfasserIn] |
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| | Davidson, Gary [VerfasserIn] |
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| Titel: | N-glycosylation of LRP6 by B3GnT2 promotes Wnt/β-Catenin signalling |
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| Verf.angabe: | Ruiyao Xu, Xianxian Wang, Sadia Safi, Nico Braunegger, Agnes Hipgrave Ederveen, Michelle Rottmann, Joachim Wittbrodt, Manfred Wuhrer, Janine Wesslowski and Gary Davidson |
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| E-Jahr: | 2023 |
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| Jahr: | 10 March 2023 |
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| Umfang: | 21 S. |
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| Fussnoten: | Gesehen am 12.06.2023 |
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| Titel Quelle: | Enthalten in: Cells |
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| Ort Quelle: | Basel : MDPI, 2012 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 12(2023), 6 vom: März, Artikel-ID 863, Seite 1-21 |
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| ISSN Quelle: | 2073-4409 |
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| Abstract: | Reception of Wnt signals by cells is predominantly mediated by Frizzled receptors in conjunction with a co-receptor, the latter being LRP6 or LRP5 for the Wnt/β-catenin signalling pathway. It is important that cells maintain precise control of receptor activation events in order to properly regulate Wnt/β-catenin signalling as aberrant signalling can result in disease in humans. Phosphorylation of the intracellular domain (ICD) of LRP6 is well known to regulate Wntβ-catenin signalling; however, less is known for regulatory post-translational modification events within the extracellular domain (ECD). Using a cell culture-based expression screen for functional regulators of LRP6, we identified a glycosyltransferase, B3GnT2-like, from a teleost fish (medaka) cDNA library, that modifies LRP6 and regulates Wnt/β-catenin signalling. We provide both gain-of-function and loss-of-function evidence that the single human homolog, B3GnT2, promotes extension of polylactosamine chains at multiple N-glycans on LRP6, thereby enhancing trafficking of LRP6 to the plasma membrane and promoting Wnt/β-catenin signalling. Our findings further highlight the importance of LRP6 as a regulatory hub in Wnt signalling and provide one of the few examples of how a specific glycosyltransferase appears to selectively target a signalling pathway component to alter cellular signalling events. |
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| DOI: | doi:10.3390/cells12060863 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.3390/cells12060863 |
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| | kostenfrei: Volltext: https://www.mdpi.com/2073-4409/12/6/863 |
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| | DOI: https://doi.org/10.3390/cells12060863 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | B3GnT2 |
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| | glycosylation |
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| | LRP6 |
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| | polylactosamine |
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| | Wnt signalling |
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| | Wnt/β-catenin |
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| K10plus-PPN: | 1848790503 |
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| Verknüpfungen: | → Zeitschrift |
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N-glycosylation of LRP6 by B3GnT2 promotes Wnt/β-Catenin signalling / Xu, Ruiyao [VerfasserIn]; 10 March 2023 (Online-Ressource)
