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| Verfasst von: | Kermani, Fatemeh [VerfasserIn]  |
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| | Mosqueira, Matias [VerfasserIn] |
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| | Peters, Kyra [VerfasserIn] |
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| | Lemma, Enrico D. [VerfasserIn] |
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| | Rapti, Kleopatra [VerfasserIn] |
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| | Grimm, Dirk [VerfasserIn] |
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| | Bastmeyer, Martin [VerfasserIn] |
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| | Laugsch, Magdalena [VerfasserIn] |
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| | Hecker, Markus [VerfasserIn] |
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| | Ullrich, Nina D. [VerfasserIn] |
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| Titel: | Membrane remodelling triggers maturation of excitation-contraction coupling in 3D-shaped human-induced pluripotent stem cell-derived cardiomyocytes |
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| Verf.angabe: | Fatemeh Kermani, Matias Mosqueira, Kyra Peters, Enrico D. Lemma, Kleopatra Rapti, Dirk Grimm, Martin Bastmeyer, Magdalena Laugsch, Markus Hecker, Nina D. Ullrich |
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| E-Jahr: | 2023 |
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| Jahr: | 29 March 2023 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 12.06.2023 |
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| Titel Quelle: | Enthalten in: Basic research in cardiology |
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| Ort Quelle: | [Darmstadt u.a.] : Steinkopff, 1937 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 118(2023) vom: März, Artikel-ID 13, Seite 1-16 |
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| ISSN Quelle: | 1435-1803 |
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| Abstract: | The prospective use of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) for cardiac regenerative medicine strongly depends on the electro-mechanical properties of these cells, especially regarding the Ca2+-dependent excitation-contraction (EC) coupling mechanism. Currently, the immature structural and functional features of hiPSC-CM limit the progression towards clinical applications. Here, we show that a specific microarchitecture is essential for functional maturation of hiPSC-CM. Structural remodelling towards a cuboid cell shape and induction of BIN1, a facilitator of membrane invaginations, lead to transverse (t)-tubule-like structures. This transformation brings two Ca2+ channels critical for EC coupling in close proximity, the L-type Ca2+ channel at the sarcolemma and the ryanodine receptor at the sarcoplasmic reticulum. Consequently, the Ca2+-dependent functional interaction of these channels becomes more efficient, leading to improved spatio-temporal synchronisation of Ca2+ transients and higher EC coupling gain. Thus, functional maturation of hiPSC-cardiomyocytes by optimised cell microarchitecture needs to be considered for future cardiac regenerative approaches. |
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| DOI: | doi:10.1007/s00395-023-00984-5 |
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| URL: | kostenfrei: Volltext: https://doi.org/10.1007/s00395-023-00984-5 |
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| | kostenfrei: Volltext: https://link.springer.com/article/10.1007/s00395-023-00984-5 |
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| | DOI: https://doi.org/10.1007/s00395-023-00984-5 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 3D reshaping |
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| | BIN1 |
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| | Excitation-contraction coupling |
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| | hiPSC cardiomyocytes |
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| | Maturation |
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| | t-tubules |
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| K10plus-PPN: | 1848795246 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Membrane remodelling triggers maturation of excitation-contraction coupling in 3D-shaped human-induced pluripotent stem cell-derived cardiomyocytes / Kermani, Fatemeh [VerfasserIn]; 29 March 2023 (Online-Ressource)
