Circulating cf-miRNA as a more appropriate surrogate liquid biopsy marker than cfDNA for ovarian cancer

• Verfasst von: Gahlawat, Aoife Ward [VerfasserIn]
• Verfasst von: Witte, Tania [VerfasserIn]
• Verfasst von: Sinn, Peter [VerfasserIn]
• Verfasst von: Schott, Sarah [VerfasserIn]
• Titel: Circulating cf-miRNA as a more appropriate surrogate liquid biopsy marker than cfDNA for ovarian cancer
• Verf.angabe: Aoife Ward Gahlawat, Tania Witte, Peter Sinn & Sarah Schott
• E-Jahr: 2023
• Jahr: 04 April 2023
• Umfang: 9 S.
• Fussnoten: Gesehen am 19.06.2023
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Macmillan Publishers Limited, part of Springer Nature, 2011
• Jahr Quelle: 2023
• Band/Heft Quelle: 13(2023) vom: Apr., Artikel-ID 5503, Seite 1-9
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/s41598-023-32243-x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: miRNAs
• Sach-SW: Molecular medicine
• Sach-SW: Ovarian cancer
• K10plus-PPN: 1850525889
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Ovarian cancer (OC) is an aggressive disease, primarily diagnosed in late stages with only 20% of patients surviving more than 5 years. Liquid biopsy markers have great potential to improve current diagnostic and prognostic methods. Here, we compared miRNAs and DNA methylation in matched plasma, whole blood and tissues as a surrogate marker for OC. We found that while both cfDNA and cf-miRNAs levels were upregulated in OC compared to patients with benign lesions or healthy controls, only cf-miRNA levels were an independent prognosticator of survival. Following on our previous work, we found members of the miR-200 family, miR-200c and miR-141 to be upregulated in both plasma and matched tissues of OC patients which correlated with adverse clinical features. We could also show that the upregulation of miR-200c and -141 correlated with promoter DNA hypomethylation in tissues, but not in plasma or matched whole blood samples. As cf-miRNAs are more easily obtained and very stable in blood, we conclude that they might serve as a more appropriate surrogate liquid biopsy marker than cfDNA for OC.