Liposomal amphotericin B eradicates Candida albicans biofilm in a continuous catheter flow model

• Verfasst von: Seidler, Marc [VerfasserIn]
• Verfasst von: Salvenmoser, Stefanie [VerfasserIn]
• Verfasst von: Müller, Frank-Michael [VerfasserIn]
• Titel: Liposomal amphotericin B eradicates Candida albicans biofilm in a continuous catheter flow model
• Verf.angabe: Marc Seidler, Stefanie Salvenmoser & Frank-Michael C. Müller
• E-Jahr: 2010
• Jahr: 24 February 2010
• Umfang: 4 S.
• Fussnoten: Gesehen am 19.06.2023
• Titel Quelle: Enthalten in: Federation of European Microbiological SocietiesFEMS yeast research
• Ort Quelle: Oxford : Oxford Univ. Press, 2001
• Jahr Quelle: 2010
• Band/Heft Quelle: 10(2010), 4, Seite 492-495
• ISSN Quelle: 1567-1364
• DOI: doi:10.1111/j.1567-1364.2010.00618.x
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1850554439

Abstract

The aim of this study was to test whether a Candida albicans biofilm can be eradicated by liposomal amphotericin B (LAMB) at the minimal inhibitory concentration in a novel catheter continuous flow model. After 24-h biofilm formation and a 24-h treatment with LAMB, the growth of the hyphal network was reduced to 20% in comparison with the untreated control, whereas fluconazole and caspofungin remained at an intermediate phase (50%). After 24-h biofilm formation and a 24-h treatment with LAMB, 20% of the surface was covered in biofilm and LAMB caused an uneven surface. For caspofungin and fluconazole, the surface covering was 80%. The extracellular matrix (ECM) of the infected, but untreated catheters had a thickness of 5-20 μm at 24 h and 10-150 μm at 48 h. After 24-h biofilm formation and a 24-h treatment with LAMB, the ECM was virtually cleared with 0 μm ECM. After 24-h biofilm formation and a 24-h treatment with fluconazole, the ECM thickness was comparable to the infected, but untreated catheter at 24 h with 10-25 μm; with caspofungin, the ECM thickness was comparable to the infected, but untreated catheter at 48 h with 10-130 μm. Comparing the blastospores, pseudohyphae and ECM, 0.5 μg mL−1 LAMB could eradicate Candida biofilm, whereas fluconazole and caspofungin were less effective.