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| Verfasst von: | Angerilli, Alessandro [VerfasserIn]  |
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| | Tait, Janet [VerfasserIn] |
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| | Berges, Julian [VerfasserIn] |
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| | Shcherbakova, Irina [VerfasserIn] |
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| | Pokrovsky, Daniil [VerfasserIn] |
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| | Schauer, Tamas [VerfasserIn] |
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| | Smialowski, Pawel [VerfasserIn] |
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| | Hsam, Ohnmar [VerfasserIn] |
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| | Mentele, Edith [VerfasserIn] |
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| | Nicetto, Dario [VerfasserIn] |
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| | Rupp, Ralph AW [VerfasserIn] |
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| Titel: | The histone H4K20 methyltransferase SUV4-20H1/KMT5B is required for multiciliated cell differentiation in Xenopus |
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| Verf.angabe: | Alessandro Angerilli, Janet Tait, Julian Berges, Irina Shcherbakova, Daniil Pokrovsky, Tamas Schauer, Pawel Smialowski, Ohnmar Hsam, Edith Mentele, Dario Nicetto, Ralph AW Rupp |
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| E-Jahr: | 2023 |
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| Jahr: | 28 April 2023 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 21.06.2023 |
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| Titel Quelle: | Enthalten in: Life science alliance |
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| Ort Quelle: | Heidelberg : EMBO Press, 2018 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 6(2023), 7 vom: Apr., Artikel-ID e202302023, Seite 1-16 |
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| ISSN Quelle: | 2575-1077 |
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| Abstract: | H4 lysine 20 dimethylation (H4K20me2) is the most abundant histone modification in vertebrate chromatin. It arises from sequential methylation of unmodified histone H4 proteins by the mono-methylating enzyme PR-SET7/KMT5A, followed by conversion to the dimethylated state by SUV4-20H (KMT5B/C) enzymes. We have blocked the deposition of this mark by depleting Xenopus embryos of SUV4-20H1/H2 methyltransferases. In the larval epidermis, this results in a severe loss of cilia in multiciliated cells (MCC), a key component of mucociliary epithelia. MCC precursor cells are correctly specified, amplify centrioles, but ultimately fail in ciliogenesis because of the perturbation of cytoplasmic processes. Genome-wide transcriptome profiling reveals that SUV4-20H1/H2-depleted ectodermal explants preferentially down-regulate the expression of several hundred ciliogenic genes. Further analysis demonstrated that knockdown of SUV4-20H1 alone is sufficient to generate the MCC phenotype and that its catalytic activity is needed for axoneme formation. Overexpression of the H4K20me1-specific histone demethylase PHF8/KDM7B also rescues the ciliogenic defect in a significant manner. Taken together, this indicates that the conversion of H4K20me1 to H4K20me2 by SUV4-20H1 is critical for the formation of cilia tufts. |
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| DOI: | doi:10.26508/lsa.202302023 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.26508/lsa.202302023 |
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| | kostenfrei: Volltext: https://www.life-science-alliance.org/content/6/7/e202302023 |
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| | DOI: https://doi.org/10.26508/lsa.202302023 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1850712697 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ histone H4K20 methyltransferase SUV4-20H1/KMT5B is required for multiciliated cell differentiation in Xenopus / Angerilli, Alessandro [VerfasserIn]; 28 April 2023 (Online-Ressource)
