Functional nuclear architecture studied by microscopy

• Verfasst von: Rouquette, Jacques [VerfasserIn]
• Verfasst von: Cremer, Christoph [VerfasserIn]
• Verfasst von: Cremer, Thomas [VerfasserIn]
• Verfasst von: Fakan, Stanislav [VerfasserIn]
• Titel: Functional nuclear architecture studied by microscopy
• Titelzusatz: present and future
• Verf.angabe: Jacques Rouquette, Christoph Cremer, Thomas Cremer, Stanislav Fakan
• E-Jahr: 2010
• Jahr: 18 June 2010
• Umfang: 90 S.
• Fussnoten: Gesehen am 22.06.2023
• Weitere Titel: Abweichender Titel: Chapter 1
• Titel Quelle: Enthalten in: International review of cell and molecular biology
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 2008
• Jahr Quelle: 2010
• Band/Heft Quelle: 282(2010), Seite 1-90
• ISSN Quelle: 1937-6448
• DOI: doi:10.1016/S1937-6448(10)82001-5
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Chromatin domain
• Sach-SW: Chromosome territory
• Sach-SW: Fluorescence microscopy
• Sach-SW: Light and electron microscopy
• Sach-SW: Nuclear functions
• Sach-SW: Perichromatin region
• Sach-SW: superresolution
• K10plus-PPN: 1850792836

Abstract

In this review we describe major contributions of light and electron microscopic approaches to the present understanding of functional nuclear architecture. The large gap of knowledge, which must still be bridged from the molecular level to the level of higher order structure, is emphasized by differences of currently discussed models of nuclear architecture. Molecular biological tools represent new means for the multicolor visualization of various nuclear components in living cells. New achievements offer the possibility to surpass the resolution limit of conventional light microscopy down to the nanometer scale and require improved bioinformatics tools able to handle the analysis of large amounts of data. In combination with the much higher resolution of electron microscopic methods, including ultrastructural cytochemistry, correlative microscopy of the same cells in their living and fixed state is the approach of choice to combine the advantages of different techniques. This will make possible future analyses of cell type- and species-specific differences of nuclear architecture in more detail and to put different models to critical tests.