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| Verfasst von: | Banović, Franjo [VerfasserIn]  |
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| | Schulze, Sandrin [VerfasserIn] |
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| | Abu Mraheil, Mobarak [VerfasserIn] |
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| | Hain, Torsten [VerfasserIn] |
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| | Chakraborty, Trinad [VerfasserIn] |
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| | Orian-Rousseau, Véronique [VerfasserIn] |
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| | Moroniak, Selina [VerfasserIn] |
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| | Weiß, Christel [VerfasserIn] |
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| | Ishikawa, Hiroshi [VerfasserIn] |
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| | Schroten, Horst [VerfasserIn] |
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| | Adam, Rüdiger [VerfasserIn] |
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| | Schwerk, Christian [VerfasserIn] |
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| Titel: | Different involvement of vimentin during invasion by listeria monocytogenes at the blood-brain and the blood-cerebrospinal fluid barriers in vitro |
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| Verf.angabe: | Franjo Banovic, Sandrin Schulze, Mobarak Abu Mraheil, Torsten Hain, Trinad Chakraborty, Véronique Orian-Rousseau, Selina Moroniak, Christel Weiss, Hiroshi Ishikawa, Horst Schroten, Rüdiger Adam and Christian Schwerk |
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| E-Jahr: | 2022 |
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| Jahr: | 26 October 2022 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 04.07.2023 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 23(2022), 21, Artikel-ID 12908, Seite 1-15 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | The human central nervous system (CNS) is separated from the blood by distinct cellular barriers, including the blood-brain barrier (BBB) and the blood-cerebrospinal fluid (CFS) barrier (BCSFB). Whereas at the center of the BBB are the endothelial cells of the brain capillaries, the BCSFB is formed by the epithelium of the choroid plexus. Invasion of cells of either the BBB or the BCSFB is a potential first step during CNS entry by the Gram-positive bacterium Listeria monocytogenes (Lm). Lm possesses several virulence factors mediating host cell entry, such as the internalin protein family—including internalin (InlA), which binds E-cadherin (Ecad) on the surface of target cells, and internalin B (InlB)—interacting with the host cell receptor tyrosine kinase Met. A further family member is internalin (InlF), which targets the intermediate filament protein vimentin. Whereas InlF has been shown to play a role during brain invasion at the BBB, its function during infection at the BCSFB is not known. We use human brain microvascular endothelial cells (HBMEC) and human choroid plexus epithelial papilloma (HIBCPP) cells to investigate the roles of InlF and vimentin during CNS invasion by Lm. Whereas HBMEC present intracellular and surface vimentin (besides Met), HIBCPP cells do not express vimentin (except Met and Ecad). Treatment with the surface vimentin modulator withaferin A (WitA) inhibited invasion of Lm into HBMEC, but not HIBCPP cells. Invasion of Lm into HBMEC and HIBCPP cells is, however, independent of InlF, since a deletion mutant of Lm lacking InlF did not display reduced invasion rates. |
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| DOI: | doi:10.3390/ijms232112908 |
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| URL: | kostenfrei: Volltext: https://doi.org/10.3390/ijms232112908 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1422-0067/23/21/12908 |
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| | DOI: https://doi.org/10.3390/ijms232112908 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | <i>Listeria monocytogenes</i> |
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| | blood-cerebrospinal fluid barrier |
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| | choroid plexus |
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| | internalin F |
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| K10plus-PPN: | 1851540601 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Different involvement of vimentin during invasion by listeria monocytogenes at the blood-brain and the blood-cerebrospinal fluid barriers in vitro / Banović, Franjo [VerfasserIn]; 26 October 2022 (Online-Ressource)
