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| Verfasst von: | Schürmann, Sebastian [VerfasserIn]  |
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| | Wegner, Frederic von [VerfasserIn] |
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| | Fink, Rainer [VerfasserIn] |
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| | Friedrich, Oliver [VerfasserIn] |
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| | Vogel, Martin [VerfasserIn] |
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| Titel: | Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils |
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| Verf.angabe: | Sebastian Schürmann, Frederic von Wegner, Rainer H.A. Fink, Oliver Friedrich, and Martin Vogel |
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| E-Jahr: | 2010 |
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| Jahr: | 21 September 2010 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 04.07.2023 |
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| Titel Quelle: | Enthalten in: Biophysical journal |
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| Ort Quelle: | Cambridge, Mass. : Cell Press, 1960 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 99(2010), 6 vom: Sept., Seite 1842-1851 |
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| ISSN Quelle: | 1542-0086 |
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| Abstract: | The second harmonic generation (SHG) signal intensity sourced from skeletal muscle myosin II strongly depends on the polarization of the incident laser beam relative to the muscle fiber axis. This dependence is related to the second-order susceptibility χ(2), which can be described by a single component ratio γ under generally assumed symmetries. We precisely extracted γ from SHG polarization dependence curves with an extended focal field model. In murine myofibrillar preparations, we have found two distinct polarization dependencies: With the actomyosin system in the rigor state, γrig has a mean value of γrig = 0.52 (SD = 0.04, n = 55); in a relaxed state where myosin is not bound to actin, γrel has a mean value of γrel = 0.24 (SD = 0.07, n = 70). We observed a similar value in an activated state where the myosin power stroke was pharmacologically inhibited using N-benzyl-p-toluene sulfonamide. In summary, different actomyosin states can be visualized noninvasively with SHG microscopy. Specifically, SHG even allows us to distinguish different actin-bound states of myosin II using γ as a parameter. |
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| DOI: | doi:10.1016/j.bpj.2010.07.005 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.bpj.2010.07.005 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0006349510008490 |
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| | DOI: https://doi.org/10.1016/j.bpj.2010.07.005 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1851563393 |
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| Verknüpfungen: | → Zeitschrift |
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Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils / Schürmann, Sebastian [VerfasserIn]; 21 September 2010 (Online-Ressource)
