| Online-Ressource |
Verfasst von: | Niaré, Karamoko [VerfasserIn]  |
| Chege, Timothy [VerfasserIn]  |
| Rosenkranz, Micha [VerfasserIn]  |
| Mwai, Kennedy [VerfasserIn]  |
| Saßmannshausen, Zoe [VerfasserIn]  |
| Odera, Dennis [VerfasserIn]  |
| Nyamako, Lydia [VerfasserIn]  |
| Tuju, James [VerfasserIn]  |
| Alfred, Tiono [VerfasserIn]  |
| Waitumbi, John N. [VerfasserIn]  |
| Ogutu, Bernhards [VerfasserIn]  |
| Sirima, Sodiomon B. [VerfasserIn]  |
| Awandare, Gordon [VerfasserIn]  |
| Kouriba, Bourema [VerfasserIn]  |
| Rayner, Julian C. [VerfasserIn]  |
| Osier, Faith [VerfasserIn]  |
Titel: | Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target |
Verf.angabe: | Karamoko Niaré, Timothy Chege, Micha Rosenkranz, Kennedy Mwai, Zoe Saßmannshausen, Dennis Odera, Lydia Nyamako, James Tuju, Tiono Alfred, John N. Waitumbi, Bernhards Ogutu, Sodiomon B. Sirima, Gordon Awandare, Bourema Kouriba, Julian C. Rayner and Faith H.A. Osier |
E-Jahr: | 2023 |
Jahr: | 14 April 2023 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 06.07.2023 |
Titel Quelle: | Enthalten in: Frontiers in immunology |
Ort Quelle: | Lausanne : Frontiers Media, 2010 |
Jahr Quelle: | 2023 |
Band/Heft Quelle: | 14(2023) vom: Apr., Artikel-ID 1156806, Seite 1-15 |
ISSN Quelle: | 1664-3224 |
Abstract: | IntroductionDetailed analyses of genetic diversity, antigenic variability, protein localization and immunological responses are vital for the prioritization of novel malaria vaccine candidates. Comprehensive approaches to determine the most appropriate antigen variants needed to provide broad protection are challenging and consequently rarely undertaken.MethodsHere, we characterized PF3D7_1136200, which we named Asparagine-Rich Merozoite Antigen (ARMA) based on the analysis of its sequence, localization and immunogenicity. We analyzed IgG and IgM responses against the common variants of ARMA in independent prospective cohort studies in Burkina Faso (N = 228), Kenya (N = 252) and Mali (N = 195) using a custom microarray, Div-KILCHIP.ResultsWe found a marked population structure between parasites from Africa and Asia. African isolates shared 34 common haplotypes, including a dominant pair although the overall selection pressure was directional (Tajima’s D = -2.57; Fu and Li’s F = -9.69; P < 0.02). ARMA was localized to the merozoite surface, IgG antibodies induced Fc-mediated degranulation of natural killer cells and strongly inhibited parasite growth in vitro. We found profound serological diversity, but IgG and IgM responses were highly correlated and a hierarchical clustering analysis identified only three major serogroups. Protective IgG and IgM antibodies appeared to target both cross-reactive and distinct epitopes across variants. However, combinations of IgG and IgM antibodies against selected variants were associated with complete protection against clinical episodes of malaria.DiscussionOur systematic strategy exploits genomic data to deduce the handful of antigen variants with the strongest potential to induce broad protection and may be broadly applicable to other complex pathogens for which effective vaccines remain elusive. |
DOI: | doi:10.3389/fimmu.2023.1156806 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://dx.doi.org/10.3389/fimmu.2023.1156806 |
| kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2023.1156806 |
| DOI: https://doi.org/10.3389/fimmu.2023.1156806 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1852084790 |
Verknüpfungen: | → Zeitschrift |
Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target / Niaré, Karamoko [VerfasserIn]; 14 April 2023 (Online-Ressource)