| |  Online-Ressource |
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| Verfasst von: | Frommhold, David [VerfasserIn]  |
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| | Kamphues, Anna [VerfasserIn] |
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| | Hepper, Ingrid [VerfasserIn] |
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| | Pruenster, Monika [VerfasserIn] |
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| | Lukic, Ivan K. [VerfasserIn] |
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| | Socher, Ines [VerfasserIn] |
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| | Zablotskaya, Victoria [VerfasserIn] |
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| | Buschmann-Prayon, Kirsten [VerfasserIn] |
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| | Lange-Sperandio, Bärbel [VerfasserIn] |
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| | Schymeinsky, Jürgen [VerfasserIn] |
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| | Ryschich, Eduard [VerfasserIn] |
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| | Pöschl, Johannes [VerfasserIn] |
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| | Kupatt, Christian [VerfasserIn] |
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| | Nawroth, Peter Paul [VerfasserIn] |
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| | Moser, Markus [VerfasserIn] |
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| | Walzog, Barbara [VerfasserIn] |
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| | Bierhaus, Angelika [VerfasserIn] |
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| | Sperandio, Markus [VerfasserIn] |
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| Titel: | RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo |
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| Verf.angabe: | David Frommhold, Anna Kamphues, Ingrid Hepper, Monika Pruenster, Ivan K. Lukić, Ines Socher, Victoria Zablotskaya, Kirsten Buschmann, Baerbel Lange-Sperandio, Jürgen Schymeinsky, Eduard Ryschich, Johannes Poeschl, Christian Kupatt, Peter P. Nawroth, Markus Moser, Barbara Walzog, Angelika Bierhaus, and Markus Sperandio |
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| E-Jahr: | 2010 |
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| Jahr: | 5 August 2010 |
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| Umfang: | 9 S. |
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| Fussnoten: | Gesehen am 11.07.2023 |
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| Titel Quelle: | Enthalten in: Blood |
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| Ort Quelle: | Washington, DC : American Society of Hematology, 1946 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 116(2010), 5, Seite 841-849 |
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| ISSN Quelle: | 1528-0020 |
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| Abstract: | The receptor for advanced glycation end products (RAGE) contributes to the inflammatory response in many acute and chronic diseases. In this context, RAGE has been identified as a ligand for the β2-integrin Mac-1 under static in vitro conditions. Because intercellular adhesion molecule (ICAM)-1 also binds β2-integrins, we studied RAGE−/−, Icam1−/−, and RAGE−/−Icam1−/− mice to define the relative contribution of each ligand for leukocyte adhesion in vivo. We show that trauma-induced leukocyte adhesion in cremaster muscle venules is strongly dependent on RAGE and ICAM-1 acting together in an overlapping fashion. Additional in vivo experiments in chimeric mice lacking endothelium-expressed RAGE and ICAM-1 located the adhesion defect to the endothelial compartment. Using microflow chambers coated with P-selectin, CXCL1, and soluble RAGE (sRAGE) demonstrated that sRAGE supports leukocyte adhesion under flow conditions in a Mac-1- but not LFA-1-dependent fashion. A static adhesion assay revealed that wild-type and RAGE−/− neutrophil adhesion and spreading were similar on immobilized sRAGE or fibrinogen. These observations indicate a crucial role of endothelium-expressed RAGE as Mac-1 ligand and uncover RAGE and ICAM-1 as a new set of functionally linked adhesion molecules, which closely cooperate in mediating leukocyte adhesion during the acute trauma-induced inflammatory response in vivo. |
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| DOI: | doi:10.1182/blood-2009-09-244293 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1182/blood-2009-09-244293 |
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| | DOI: https://doi.org/10.1182/blood-2009-09-244293 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1852350032 |
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| Verknüpfungen: | → Zeitschrift |
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RAGE and ICAM-1 cooperate in mediating leukocyte recruitment during acute inflammation in vivo / Frommhold, David [VerfasserIn]; 5 August 2010 (Online-Ressource)
