Arp1, an actin-related protein, in Plasmodium berghei

• Verfasst von: Siden-Kiamos, Inga [VerfasserIn]
• Verfasst von: Schüler, Herwig [VerfasserIn]
• Verfasst von: Liakopoulos, Dimitris [VerfasserIn]
• Verfasst von: Louis, Christos [VerfasserIn]
• Titel: Arp1, an actin-related protein, in Plasmodium berghei
• Verf.angabe: Inga Siden-Kiamos, Herwig Schüler, Dimitris Liakopoulos, Christos Louis
• E-Jahr: 2010
• Jahr: 16 May 2010
• Umfang: 9 S.
• Fussnoten: Gesehen am 19.07.2023
• Titel Quelle: Enthalten in: Molecular and biochemical parasitology
• Ort Quelle: Amsterdam : Elsevier, 1980
• Jahr Quelle: 2010
• Band/Heft Quelle: 173(2010), 2, Seite 88-96
• ISSN Quelle: 1872-9428
• DOI: doi:10.1016/j.molbiopara.2010.05.008
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Dynactin
• Sach-SW: Malaria
• Sach-SW: Ookinete
• Sach-SW: Yeast
• K10plus-PPN: 1853079316

Abstract

Actin-related proteins (Arps) constitute a family of eukaryotic cytoskeletal proteins involved in such diverse events as cell motility, cytokinesis, vesicle transport, and chromatin remodelling. Previously, in a study of Plasmodium berghei gene expression in ookinetes and oocysts, we detected stage-specific increased expression of a gene encoding an Arp. Here we further characterize this gene and the encoded protein. We present a phylogenetic and three-dimensional modelling analysis as well as cell biological and genetic data that support classification of this gene as being an orthologue of the actin-related protein 1 (Arp1). This gene was found to be expressed in asexual stages as well as in the mosquito stages of the parasite, both on the transcript and protein level. Our attempts to delete the gene in the parasite for functional studies were unsuccessful, suggesting that it may be essential. The protein was localized apically of the nucleus in ookinetes, and in combination with the known function of Arp1 proteins, this suggests a role in vesicular transport. Expression of the gene in Saccharomyces cerevisiae resulted in toxic effects and interference with the yeast cytoskeleton.