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Verfasst von:Golani, Gonen [VerfasserIn]   i
 Schwarz, Ulrich S. [VerfasserIn]   i
Titel:High curvature promotes fusion of lipid membranes
Titelzusatz:Predictions from continuum elastic theory
Verf.angabe:Gonen Golani and Ulrich S. Schwarz
E-Jahr:2023
Jahr:16 May 2023
Umfang:15 S.
Illustrationen:Illustrationen
Fussnoten:Online verfügbar 18. April 2023, Artikelversion 16. Mai 2023 ; Gesehen am 21.07.2023
Titel Quelle:Enthalten in: Biophysical journal
Ort Quelle:Cambridge, Mass. : Cell Press, 1960
Jahr Quelle:2023
Band/Heft Quelle:122(2023), 10 vom: Mai, Seite 1868-1882
ISSN Quelle:1542-0086
Abstract:The fusion of lipid membranes progresses through a series of hemifusion intermediates with two significant energy barriers related to the formation of stalk and fusion pore, respectively. These energy barriers determine the speed and success rate of many critical biological processes, including the fusion of highly curved membranes, for example synaptic vesicles and enveloped viruses. Here we use continuum elastic theory of lipid monolayers to determine the relationship between membrane shape and energy barriers to fusion. We find that the stalk formation energy decreases with curvature by up to 31 kBT in a 20-nm-radius vesicle compared with planar membranes and by up to 8 kBT in the fusion of highly curved, long, tubular membranes. In contrast, the fusion pore formation energy barrier shows a more complicated behavior. Immediately after stalk expansion to the hemifusion diaphragm, the fusion pore formation energy barrier is low (15-25 kBT) due to lipid stretching in the distal monolayers and increased tension in highly curved vesicles. Therefore, the opening of the fusion pore is faster. However, these stresses relax over time due to lipid flip-flop from the proximal monolayer, resulting in a larger hemifusion diaphragm and a higher fusion pore formation energy barrier, up to 35 kBT. Therefore, if the fusion pore fails to open before significant lipid flip-flop takes place, the reaction proceeds to an extended hemifusion diaphragm state, which is a dead-end configuration in the fusion process and can be used to prevent viral infections. In contrast, in the fusion of long tubular compartments, the surface tension does not accumulate due to the formation of the diaphragm, and the energy barrier for pore expansion increases with curvature by up to 11 kBT. This suggests that inhibition of polymorphic virus infection could particularly target this feature of the second barrier.
DOI:doi:10.1016/j.bpj.2023.04.018
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1016/j.bpj.2023.04.018
 Volltext: https://www.sciencedirect.com/science/article/pii/S0006349523002692
 DOI: https://doi.org/10.1016/j.bpj.2023.04.018
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1853238864
Verknüpfungen:→ Zeitschrift

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