Histological comparative analysis of Bowman layer grafts procured using 3 different techniques

• Verfasst von: Son, Hyeck Soo [VerfasserIn]
• Verfasst von: Moon, Loren [VerfasserIn]
• Verfasst von: Wang, Jiangxia [VerfasserIn]
• Verfasst von: Eberhart, Charles G. [VerfasserIn]
• Verfasst von: Jun, Albert S. [VerfasserIn]
• Verfasst von: Srikumaran, Divya [VerfasserIn]
• Verfasst von: Soiberman, Uri S. [VerfasserIn]
• Titel: Histological comparative analysis of Bowman layer grafts procured using 3 different techniques
• Verf.angabe: Hyeck-Soo Son, Loren Moon, Jiangxia Wang, Charles G. Eberhart, Albert S. Jun, Divya Srikumaran, Uri S. Soiberman
• E-Jahr: 2023
• Jahr: July 2023
• Umfang: 6 S.
• Fussnoten: Gesehen am 24.07.2023
• Titel Quelle: Enthalten in: Cornea
• Ort Quelle: Philadelphia, Pa. : Lippincott, Williams & Wilkins, 1982
• Jahr Quelle: 2023
• Band/Heft Quelle: 42(2023), 7 vom: Juli, Seite 888-893
• ISSN Quelle: 1536-4798
• DOI: doi:10.1097/ICO.0000000000003260
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1853474398

Abstract

Purpose: - The objective of this study is to perform a histological analysis of Bowman layer (BL) grafts. - Methods: - BL grafts were procured from 13 human cadaver corneal tissues using 3 different donor preparation techniques. Subsequently, the grafts were fixed in 10% buffered formalin phosphate and embedded in paraffin. Hematoxylin and eosin sections of BL grafts were obtained and analyzed under a light microscope. BL and full graft thickness were measured using an image-processing software. - Results: - All 13 BL grafts contained residual anterior stromal tissue. BL stripping using Kelman-McPherson and Moorfield forceps (technique 3) achieved the thinnest graft thickness with a mean full graft thickness of 18.7 μm (95% confidence interval [CI], −9.8 to 47.2) at the thinnest point of the graft, whereas BL procurement using the Melles lamellar dissector (technique 2) led to the highest mean full graft thickness of 279.9 μm (95% CI, 251.4-308.5) even at the thinnest area of the graft. By contrast, BL dissection using a blunt dissector (technique 1) provided a mean full graft thickness of 70.2 μm (95% CI, 40.4-100.1) at the graft's thinnest point. Although peripheral graft tears occurred in 50%, 50%, and 100% of techniques 1, 2, and 3, respectively, intact 6.25-mm diameter BL grafts could be secured in 50%, 100%, and 80% of techniques 1, 2, and 3, respectively. - Conclusions: - None of the techniques used led to the procurement of pure BL grafts devoid of the anterior stroma. Peripheral scoring with a thin needle and tissue manipulation with Kelman-McPherson and Moorfield forceps led to the thinnest grafts in this study.