Mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene

• Verfasst von: Galovic, Vladislava [VerfasserIn]
• Verfasst von: Rausch, Thomas [VerfasserIn]
• Verfasst von: Grsic-Rausch, Slobodanka [VerfasserIn]
• Titel: Mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene
• Verf.angabe: Vladislava Galović, T. Rausch and Slobodanka Gršić-Rausch
• Jahr: 2010
• Umfang: 8 S.
• Fussnoten: Gesehen am 25.07.2023
• Titel Quelle: Enthalten in: Archives of biological sciences
• Ort Quelle: Belgrade : Srpsko Biološko Društvo, 2002
• Jahr Quelle: 2010
• Band/Heft Quelle: 62(2010), 3, Seite 539-546
• ISSN Quelle: 1821-4339
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Galovic, Vladislava: Mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene. - 2010
• K10plus-PPN: 1853545422

Abstract

The mature embryos of fourteen elite winter wheat cultivars have been transformed by a biolistic approach. The gene coding for γ-glutamylcysteine synthetase (EC 6.3.2.2) was used as a transgene in order to obtain stable transformants resistant to drought stress. A binary vector, pBinarUTRECS, was used. The gene was under the control of the CaMV35S promoter region. GUS::GFP gene fusion was used as a reporter system and nptII served as a selectable marker gene. A high regeneration capacity of callus tissue under the selective pressure and successful GUS assay of transformed tissue were an indication of successful insertion of a transgene into mature embryo derived wheat tissue. .