Crystal violet staining is a reliable alternative to bicinchoninic acid assay-based normalization

• Verfasst von: Nilles, Julie [VerfasserIn]
• Verfasst von: Weiß, Johanna [VerfasserIn]
• Verfasst von: Theile, Dirk [VerfasserIn]
• Titel: Crystal violet staining is a reliable alternative to bicinchoninic acid assay-based normalization
• Verf.angabe: Julie Nilles, Johanna Weiss & Dirk Theile
• E-Jahr: 2022
• Jahr: 24 August 2022
• Umfang: 5 S.
• Fussnoten: Gesehen am 28.07.2023
• Titel Quelle: Enthalten in: BioTechniques
• Ort Quelle: London, UK : Future Science Ltd, 1983
• Jahr Quelle: 2022
• Band/Heft Quelle: 73(2022), 3, Seite 131-135
• ISSN Quelle: 1940-9818
• DOI: doi:10.2144/btn-2022-0064
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: alternative
• Sach-SW: bicinchoninic acid assay
• Sach-SW: crystal violet
• Sach-SW: protein content
• K10plus-PPN: 1853837741
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Experimental data with cells often require normalization. The frequently used bicinchoninic acid (BCA) assay, in fact, indicates protein content but is influenced by incubation time, pH etc. A simple, rapid and reliable alternative is desirable. Crystal violet stains nucleic acids and proteins and was used to reflect the cell number in 96-well plates. Calibration curves and comparison with BCA confirmed excellent goodness of fit (R2: 0.98), conformity (nonsignificant difference of BCA to crystal violet) and reliability of this staining methodology. Crystal violet staining can be used to normalize experimental data to the number of adherent cells present in cell culture plates.