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| Verfasst von: | Bessler, Larissa Sabine [VerfasserIn]  |
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| | Kaur, Navpreet [VerfasserIn] |
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| | Vogt, Lea-Marie [VerfasserIn] |
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| | Flemmich, Laurin [VerfasserIn] |
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| | Siebenaller, Carmen [VerfasserIn] |
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| | Winz, Marie-Luise [VerfasserIn] |
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| | Tuorto, Francesca [VerfasserIn] |
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| | Micura, Ronald [VerfasserIn] |
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| | Ehrenhofer-Murray, Ann E. [VerfasserIn] |
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| | Helm, Mark [VerfasserIn] |
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| Titel: | Functional integration of a semi-synthetic azido-queuosine derivative into translation and a tRNA modification circuit |
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| Verf.angabe: | Larissa Bessler, Navpreet Kaur, Lea-Marie Vogt, Laurin Flemmich, Carmen Siebenaller, Marie-Luise Winz, Francesca Tuorto, Ronald Micura, Ann E. Ehrenhofer-Murray and Mark Helm |
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| E-Jahr: | 2022 |
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| Jahr: | 28 September 2022 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 01.08.2023 |
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| Titel Quelle: | Enthalten in: Nucleic acids research |
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| Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 50(2022), 18 vom: Okt., Seite 10785-10800 |
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| ISSN Quelle: | 1362-4962 |
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| Abstract: | Substitution of the queuine nucleobase precursor preQ1 by an azide-containing derivative (azido-propyl-preQ1) led to incorporation of this clickable chemical entity into tRNA via transglycosylation in vitro as well as in vivo in Escherichia coli, Schizosaccharomyces pombe and human cells. The resulting semi-synthetic RNA modification, here termed Q-L1, was present in tRNAs on actively translating ribosomes, indicating functional integration into aminoacylation and recruitment to the ribosome. The azide moiety of Q-L1 facilitates analytics via click conjugation of a fluorescent dye, or of biotin for affinity purification. Combining the latter with RNAseq showed that TGT maintained its native tRNA substrate specificity in S. pombe cells. The semi-synthetic tRNA modification Q-L1 was also functional in tRNA maturation, in effectively replacing the natural queuosine in its stimulation of further modification of tRNAAsp with 5-methylcytosine at position 38 by the tRNA methyltransferase Dnmt2 in S. pombe. This is the first demonstrated in vivo integration of a synthetic moiety into an RNA modification circuit, where one RNA modification stimulates another. In summary, the scarcity of queuosinylation sites in cellular RNA, makes our synthetic q/Q system a 'minimally invasive' system for placement of a non-natural, clickable nucleobase within the total cellular RNA. |
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| DOI: | doi:10.1093/nar/gkac822 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1093/nar/gkac822 |
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| | kostenfrei: Volltext: https://academic.oup.com/nar/article/50/18/10785/6725769?login=true |
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| | DOI: https://doi.org/10.1093/nar/gkac822 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | 5-Methylcytosine |
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| | Azides |
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| | Biotin |
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| | Escherichia coli |
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| | Fluorescent Dyes |
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| | Humans |
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| | Nucleoside Q |
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| | RNA, Transfer |
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| | RNA, Transfer, Asp |
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| | Schizosaccharomyces |
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| | tRNA Methyltransferases |
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| K10plus-PPN: | 1853995738 |
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| Verknüpfungen: | → Zeitschrift |
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Functional integration of a semi-synthetic azido-queuosine derivative into translation and a tRNA modification circuit / Bessler, Larissa Sabine [VerfasserIn]; 28 September 2022 (Online-Ressource)
