A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco

• Verfasst von: Dalakouras, Athanasios [VerfasserIn]
• Verfasst von: Moser, Mirko [VerfasserIn]
• Verfasst von: Krczal, Gabi [VerfasserIn]
• Verfasst von: Wassenegger, Michael [VerfasserIn]
• Titel: A chimeric satellite transgene sequence is inefficiently targeted by viroid-induced DNA methylation in tobacco
• Verf.angabe: Athanasios Dalakouras, Mirko Moser, Gabi Krczal, Michael Wassenegger
• E-Jahr: 2010
• Jahr: 03 April 2010
• Umfang: 9 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 02.08.2023
• Titel Quelle: Enthalten in: Plant molecular biology
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V, 1981
• Jahr Quelle: 2010
• Band/Heft Quelle: 73(2010), 4/5 vom: Juli, Seite 439-447
• ISSN Quelle: 1573-5028
• DOI: doi:10.1007/s11103-010-9631-6
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Base Sequence
• Sach-SW: Blotting, Northern
• Sach-SW: Blotting, Southern
• Sach-SW: DNA Methylation
• Sach-SW: DNA, Recombinant
• Sach-SW: Gene Expression Regulation, Plant
• Sach-SW: Gene Silencing
• Sach-SW: Molecular Sequence Data
• Sach-SW: Plant Viruses
• Sach-SW: RNA Stability
• Sach-SW: Sequence Analysis, DNA
• Sach-SW: Tobacco
• Sach-SW: Tobacco mosaic satellite virus
• Sach-SW: Transgenes
• Sach-SW: Viroids
• K10plus-PPN: 1854201557

Abstract

In plants, transgenes containing Potato spindle tuber viroid (PSTVd) cDNA sequences were efficient targets of PSTVd infection-mediated RNA-directed DNA methylation. Here, we demonstrate that in PSTVd-infected tobacco plants, a 134 bp PSTVd fragment (PSTVd-134) did not become densely methylated when it was inserted into a chimeric Satellite tobacco mosaic virus (STMV) construct. Only about 4-5% of all cytosines (Cs) of the PSTVd-134 were methylated when flanked by satellite sequences. In the same plants, C methylation was approximately 92% when the PSTVd-134 was in a PSTVd full length sequence context and roughly 33% when flanked at its 3' end by a 19 bp PSTVd and at its 5' end by a short viroid-unrelated sequence. In addition, PSTVd small interfering RNAs (siRNAs) produced from the replicating viroid failed to target PSTVd-134-containing chimeric STMV RNA for degradation. Satellite RNAs appear to have adopted secondary structures that protect them against RNA interference (RNAi)-mediated degradation. Protection can be extended to short non-satellite sequences residing in satellite RNAs, rendering them poor targets for nuclear and cytoplasmic RNAi induced in trans.