Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells

• Verfasst von: Schaaf, Christian P. [VerfasserIn]
• Verfasst von: Shan, Bing [VerfasserIn]
• Verfasst von: Onofri, Chiara [VerfasserIn]
• Verfasst von: Stalla, Günter K. [VerfasserIn]
• Verfasst von: Arzt, Eduardo [VerfasserIn]
• Verfasst von: Schilling, Tobias [VerfasserIn]
• Verfasst von: Perone, Marcelo J. [VerfasserIn]
• Verfasst von: Renner, Ulrich [VerfasserIn]
• Titel: Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
• Verf.angabe: Christian Schaaf, Bing Shan, Chiara Onofri, Günter K. Stalla, Eduardo Arzt, Tobias Schilling, Marcelo J. Perone, Ulrich Renner
• E-Jahr: 2010
• Jahr: February 17 2010
• Umfang: 11 S.
• Fussnoten: Gesehen am 08.08.2023
• Titel Quelle: Enthalten in: Neuroendocrinology
• Ort Quelle: Basel : Karger, 1965
• Jahr Quelle: 2010
• Band/Heft Quelle: 91(2010), 2, Seite 200-210
• ISSN Quelle: 1423-0194
• DOI: doi:10.1159/000287236
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1854789716

Abstract

The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-ĸB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis.