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| Verfasst von: | Gelléri, Márton [VerfasserIn]  |
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| | Chen, Shih-Ya [VerfasserIn] |
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| | Hübner, Barbara [VerfasserIn] |
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| | Neumann, Jan [VerfasserIn] |
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| | Kröger, Ole [VerfasserIn] |
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| | Sadlo, Filip [VerfasserIn] |
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| | Imhoff, Jorg [VerfasserIn] |
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| | Hendzel, Michael J. [VerfasserIn] |
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| | Cremer, Marion [VerfasserIn] |
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| | Cremer, Thomas [VerfasserIn] |
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| | Strickfaden, Hilmar [VerfasserIn] |
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| | Cremer, Christoph [VerfasserIn] |
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| Titel: | True-to-scale DNA-density maps correlate with major accessibility differences between active and inactive chromatin |
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| Verf.angabe: | Márton Gelléri, Shih-Ya Chen, Barbara Hübner, Jan Neumann, Ole Kröger, Filip Sadlo, Jorg Imhoff, Michael J. Hendzel, Marion Cremer, Thomas Cremer, Hilmar Strickfaden, Christoph Cremer |
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| E-Jahr: | 2023 |
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| Jahr: | 27 June 2023 |
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| Umfang: | 22 S. |
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| Illustrationen: | Illustrationen |
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| Fussnoten: | Online verfügbar: 26. Mai 2023 ; Gesehen am 14.08.2023 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2023 |
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| Band/Heft Quelle: | 42(2023), 6 vom: Juni, Artikel-ID 112567, Seite 1-22 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Chromatin compaction differences may have a strong impact on accessibility of individual macromolecules and macromolecular assemblies to their DNA target sites. Estimates based on fluorescence microscopy with conventional resolution, however, suggest only modest compaction differences (∼2-10×) between the active nuclear compartment (ANC) and inactive nuclear compartment (INC). Here, we present maps of nuclear landscapes with true-to-scale DNA densities, ranging from <5 to >300 Mbp/μm3. Maps are generated from individual human and mouse cell nuclei with single-molecule localization microscopy at ∼20 nm lateral and ∼100 nm axial optical resolution and are supplemented by electron spectroscopic imaging. Microinjection of fluorescent nanobeads with sizes corresponding to macromolecular assemblies for transcription into nuclei of living cells demonstrates their localization and movements within the ANC and exclusion from the INC. |
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| DOI: | doi:10.1016/j.celrep.2023.112567 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.celrep.2023.112567 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2211124723005788 |
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| | DOI: https://doi.org/10.1016/j.celrep.2023.112567 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | active and inactive nuclear compartments |
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| | ANC |
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| | electron spectroscopic imaging |
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| | ESI |
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| | INC |
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| | microinjected nanobeads |
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| | quantitative image analysis |
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| | single molecule localization microscopy |
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| | SMLM |
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| | true-to-scale DNA density maps |
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| | Voronoi tessellation |
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| K10plus-PPN: | 1856105954 |
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| Verknüpfungen: | → Zeitschrift |
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True-to-scale DNA-density maps correlate with major accessibility differences between active and inactive chromatin / Gelléri, Márton [VerfasserIn]; 27 June 2023 (Online-Ressource)
